Anti-EPB41L3/DAL1 antibody (ab154071)
Key features and details
- Rabbit polyclonal to EPB41L3/DAL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EPB41L3/DAL1 antibody -
Description
Rabbit polyclonal to EPB41L3/DAL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EPB41L3/DAL1 aa 921-1087.
Database link: Q9Y2J2 -
Positive control
- PC3 and U-87 MG whole cell lysates; HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 120 kDa.
|
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 120 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
Critical growth regulator in the pathogenesis of meningiomas. -
Tissue specificity
Expressed at high levels in brain, with lower levels in kidney, intestine, and testis. -
Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 23136 Human
- Omim: 605331 Human
- SwissProt: Q9Y2J2 Human
- Unigene: 213394 Human
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Alternative names
- 4.1B antibody
- Band 4.1 like protein 3 antibody
- Band 4.1-like protein 3 antibody
see all
Images
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Western blot - Anti-EPB41L3/DAL1 antibody (ab154071)All lanes : Anti-EPB41L3/DAL1 antibody (ab154071) at 1/1000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : U-87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 120 kDa
5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-EPB41L3/DAL1 antibody (ab154071)Confocal immunofluorescent analysis of methanol-fixed HepG2 (ice-cold; 5 mins) cells labeling EPB41L3/DAL1 with ab154071 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L3/DAL1 antibody (ab154071)Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling EPB41L3/DAL1 with ab154071 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab154071 has been referenced in 5 publications.
- He X & Shu Y miR-452 promotes the development of gastric cancer via targeting EPB41L3. Pathol Res Pract 216:152725 (2020). PubMed: 31734055
- Hou A et al. LncRNA MAGI2-AS3 Affects Cell Invasion and Migration of Cervical Squamous Cell Carcinoma (CSCC) via Sponging miRNA-233/EPB41L3 Axis. Cancer Manag Res 12:4209-4216 (2020). PubMed: 32581592
- Yuan X et al. Erythrocyte membrane protein band 4.1-like 3 inhibits osteosarcoma cell invasion through regulation of Snai1-induced epithelial-to-mesenchymal transition. Aging (Albany NY) 13:1947-1961 (2020). PubMed: 33323539
- Guo H et al. Decreased expression levels of DAL-1 and TOB1 are associated with clinicopathological features and poor prognosis in gastric cancer. Pathol Res Pract N/A:152403 (2019). PubMed: 30962003
- Cai T et al. MicroRNA-26a regulates ANXA1, rather than DAL-1, in the development of lung cancer. Oncol Lett 15:5893-5902 (2018). PubMed: 29552220