Anti-EPB41L3/DAL1 antibody (ab154071)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-EPB41L3/DAL1 antibody
See all EPB41L3/DAL1 primary antibodies -
Description
Rabbit polyclonal to EPB41L3/DAL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EPB41L3/DAL1 aa 921-1087.
Database link: Q9Y2J2 -
Positive control
- PC3 and U-87 MG whole cell lysates; HepG2 cells.
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General notes
Previously labelled as EPB41L3.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 120 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Critical growth regulator in the pathogenesis of meningiomas. -
Tissue specificity
Expressed at high levels in brain, with lower levels in kidney, intestine, and testis. -
Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 23136 Human
- Omim: 605331 Human
- SwissProt: Q9Y2J2 Human
- Unigene: 213394 Human
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Alternative names
- 4.1B antibody
- Band 4.1 like protein 3 antibody
- Band 4.1-like protein 3 antibody
see all
Images
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Western blot - Anti-EPB41L3/DAL1 antibody (ab154071)All lanes : Anti-EPB41L3/DAL1 antibody (ab154071) at 1/1000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : U-87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 120 kDa
5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-EPB41L3/DAL1 antibody (ab154071)Confocal immunofluorescent analysis of methanol-fixed HepG2 (ice-cold; 5 mins) cells labeling EPB41L3/DAL1 with ab154071 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L3/DAL1 antibody (ab154071)Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling EPB41L3/DAL1 with ab154071 at 1/100 dilution.
Protocols
References
This product has been referenced in:
- Guo H et al. Decreased expression levels of DAL-1 and TOB1 are associated with clinicopathological features and poor prognosis in gastric cancer. Pathol Res Pract N/A:152403 (2019). Read more (PubMed: 30962003) »
- Cai T et al. MicroRNA-26a regulates ANXA1, rather than DAL-1, in the development of lung cancer. Oncol Lett 15:5893-5902 (2018). Read more (PubMed: 29552220) »
