Anti-EPB41L3/DAL1 antibody (ab154071)
Key features and details
- Rabbit polyclonal to EPB41L3/DAL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-EPB41L3/DAL1 antibody -
Description
Rabbit polyclonal to EPB41L3/DAL1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EPB41L3/DAL1 aa 921-1087.
Database link: Q9Y2J2 -
Positive control
- PC3 and U-87 MG whole cell lysates; HepG2 cells.
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General notes
Previously labelled as EPB41L3.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 120 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Critical growth regulator in the pathogenesis of meningiomas. -
Tissue specificity
Expressed at high levels in brain, with lower levels in kidney, intestine, and testis. -
Sequence similarities
Contains 1 FERM domain. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 23136 Human
- Omim: 605331 Human
- SwissProt: Q9Y2J2 Human
- Unigene: 213394 Human
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Alternative names
- 4.1B antibody
- Band 4.1 like protein 3 antibody
- Band 4.1-like protein 3 antibody
see all
Images
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Western blot - Anti-EPB41L3/DAL1 antibody (ab154071)All lanes : Anti-EPB41L3/DAL1 antibody (ab154071) at 1/1000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : U-87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 120 kDa
5 % SDS-PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-EPB41L3/DAL1 antibody (ab154071)Confocal immunofluorescent analysis of methanol-fixed HepG2 (ice-cold; 5 mins) cells labeling EPB41L3/DAL1 with ab154071 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EPB41L3/DAL1 antibody (ab154071)Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling EPB41L3/DAL1 with ab154071 at 1/100 dilution.
Protocols
References (2)
ab154071 has been referenced in 2 publications.
- Guo H et al. Decreased expression levels of DAL-1 and TOB1 are associated with clinicopathological features and poor prognosis in gastric cancer. Pathol Res Pract N/A:152403 (2019). PubMed: 30962003
- Cai T et al. MicroRNA-26a regulates ANXA1, rather than DAL-1, in the development of lung cancer. Oncol Lett 15:5893-5902 (2018). PubMed: 29552220
