Key features and details
- Rabbit polyclonal to EPB41L3/DAL1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-EPB41L3/DAL1 antibody
DescriptionRabbit polyclonal to EPB41L3/DAL1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human EPB41L3/DAL1 aa 921-1087.
Database link: Q9Y2J2
- PC3 and U-87 MG whole cell lysates; HepG2 cells.
Previously labelled as EPB41L3.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154071 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 120 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
FunctionCritical growth regulator in the pathogenesis of meningiomas.
Tissue specificityExpressed at high levels in brain, with lower levels in kidney, intestine, and testis.
Sequence similaritiesContains 1 FERM domain.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- 4.1B antibody
- Band 4.1 like protein 3 antibody
- Band 4.1-like protein 3 antibody
All lanes : Anti-EPB41L3/DAL1 antibody (ab154071) at 1/1000 dilution
Lane 1 : PC3 whole cell lysate
Lane 2 : U-87 MG whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 120 kDa
5 % SDS-PAGE
Confocal immunofluorescent analysis of methanol-fixed HepG2 (ice-cold; 5 mins) cells labeling EPB41L3/DAL1 with ab154071 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human A549 xenograft tissue, labeling EPB41L3/DAL1 with ab154071 at 1/100 dilution.
ab154071 has been referenced in 2 publications.
- Guo H et al. Decreased expression levels of DAL-1 and TOB1 are associated with clinicopathological features and poor prognosis in gastric cancer. Pathol Res Pract N/A:152403 (2019). PubMed: 30962003
- Cai T et al. MicroRNA-26a regulates ANXA1, rather than DAL-1, in the development of lung cancer. Oncol Lett 15:5893-5902 (2018). PubMed: 29552220