Product nameAnti-EPB4IL2 antibody [EPR8873(2)]
See all EPB4IL2 primary antibodies
DescriptionRabbit monoclonal [EPR8873(2)] to EPB4IL2
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human EPB4IL2 aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O43491
- C6, Hela, SH-SY5Y, HepG2 and fetal brain lysates. Human uterus tissue, HepG2 cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab175928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 113 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
Tissue specificityWidely expressed.
Sequence similaritiesContains 1 FERM domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
- 4.1 G antibody
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Immunohistochemical analysis of paraffin-embedded Human uterus tissue labeling EPB4IL2 with ab17928 at 1/50 dilution.
All lanes : Anti-EPB4IL2 antibody [EPR8873(2)] (ab175928) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : Hela cell lysate
Lane 3 : SH-SH5Y cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 113 kDa
Immunofluorescent staining of HepG2 cells using labeling EPB41L2 with ab175928 at 1/100 dilution (green). DAPI nuclear staining (blue).
Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab175928 at 1/10 dilution
ab175928 has not yet been referenced specifically in any publications.