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    epc1-antibody-ab112043.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-EPC1 antibody (ab112043)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

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Western blot - Anti-EPC1 antibody (ab112043)
  • Immunoprecipitation - Anti-EPC1 antibody (ab112043)

Key features and details

  • Rabbit polyclonal to EPC1
  • Suitable for: WB, IP
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-EPC1 antibody
  • Description

    Rabbit polyclonal to EPC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 325-375 of Human EPC1 (NP_079485.1).

  • Positive control

    • HeLa whole cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HAT

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab112043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
IP
Use at 2-10 µg/mg of lysate.
Notes
WB
1/2000 - 1/10000. Predicted molecular weight: 93 kDa.
IP
Use at 2-10 µg/mg of lysate.

Target

  • Function

    Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage.
  • Sequence similarities

    Belongs to the enhancer of polycomb family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q9H2F5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 450393 Chimpanzee
    • Entrez Gene: 80314 Human
    • Entrez Gene: 13831 Mouse
    • Entrez Gene: 100362678 Rat
    • Omim: 610999 Human
    • SwissProt: Q9H2F5 Human
    • SwissProt: Q8C9X6 Mouse
    • Unigene: 167805 Human
    • Unigene: 720416 Human
    • Unigene: 722439 Human
    • Unigene: 312133 Mouse
    see all
  • Alternative names

    • DKFZp781P2312 antibody
    • Enhancer of polycomb 1 antibody
    • Enhancer of polycomb homolog 1 (Drosophila) antibody
    • Enhancer of polycomb homolog 1 antibody
    • EPC1 antibody
    • EPC1_HUMAN antibody
    • Epl 1 antibody
    • Epl1 antibody
    • OTTHUMP00000046260 antibody
    see all

Images

  • Western blot - Anti-EPC1 antibody (ab112043)
    Western blot - Anti-EPC1 antibody (ab112043)
    All lanes : Anti-EPC1 antibody (ab112043) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique.

    Predicted band size: 93 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation - Anti-EPC1 antibody (ab112043)
    Immunoprecipitation - Anti-EPC1 antibody (ab112043)
    Detection of EPC1 by Western Blot of Immunprecipitate.
    ab112043 at 1µg/ml staining EPC1 in HeLa whole cell lysate immunoprecipitated using ab112043 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (1)

Publishing research using ab112043? Please let us know so that we can cite the reference in this datasheet.

ab112043 has been referenced in 1 publication.

  • Wang Y  et al. Epigenetic factor EPC1 is a master regulator of DNA damage response by interacting with E2F1 to silence death and activate metastasis-related gene signatures. Nucleic Acids Res 44:117-33 (2016). ChIP . PubMed: 26350215

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