Anti-EPC1 antibody (ab112043)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-EPC1 antibody
See all EPC1 primary antibodies -
DescriptionRabbit polyclonal to EPC1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 325-375 of Human EPC1 (NP_079485.1).
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Positive control
- HeLa whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.50
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab112043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 93 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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FunctionComponent of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage.
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Sequence similaritiesBelongs to the enhancer of polycomb family.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 450393 Chimpanzee
- Entrez Gene: 80314 Human
- Entrez Gene: 13831 Mouse
- Entrez Gene: 100362678 Rat
- Omim: 610999 Human
- SwissProt: Q9H2F5 Human
- SwissProt: Q8C9X6 Mouse
- Unigene: 167805 Human
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Alternative names
- DKFZp781P2312 antibody
- Enhancer of polycomb 1 antibody
- Enhancer of polycomb homolog 1 (Drosophila) antibody
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Images
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Western blot - Anti-EPC1 antibody (ab112043)All lanes : Anti-EPC1 antibody (ab112043) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-EPC1 antibody (ab112043)Detection of EPC1 by Western Blot of Immunprecipitate.
ab112043 at 1µg/ml staining EPC1 in HeLa whole cell lysate immunoprecipitated using ab112043 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.
References
This product has been referenced in:
- Wang Y et al. Epigenetic factor EPC1 is a master regulator of DNA damage response by interacting with E2F1 to silence death and activate metastasis-related gene signatures. Nucleic Acids Res 44:117-33 (2016). ChIP . Read more (PubMed: 26350215) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"