Product nameAnti-EPC1 antibody
See all EPC1 primary antibodies
DescriptionRabbit polyclonal to EPC1
Tested applicationsSuitable for: ICC/IF, ICC, WBmore details
Species reactivityReacts with: Human
- This antibody gave a positive signal in the following whole cell lysates: HeLa, Hek293, U2OS
General notesFrom initial efforts, this antibody does not appear to work convincingly in Western Blot. Collaborative data has also found that this ab does not work in WB even with an overexpressed flagged form of EPC1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab5514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
FunctionComponent of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage.
Sequence similaritiesBelongs to the enhancer of polycomb family.
- Information by UniProt
- DKFZp781P2312 antibody
- Enhancer of polycomb 1 antibody
- Enhancer of polycomb homolog 1 (Drosophila) antibody
Specificity of ab5514 against EPC1
A and B: Detection using indirest immunofluorescence of the signal corresponding to EPC1 in EPC1 in 293 Cells were fixed using 4% formaldehyde blocked with PBS containing 3% milk and 0.5% Triton x-100 (sigma) incubated for 1 hour at 37 C with ab5514 at a 1/50 dilution. The cells were then washed three times and incubated for 1 hour at 37 C with a donkey anti-rabbit antibody coupled with Alexa Fluor 488 (Molecular probes) at a 1/500 dilution, washed three times, stained with DAPI and mounted with Vectasheild(Abcyss). A: Acquisition on a CCD camera B: Confocal microscopy (3um stack along Z axis).
Verification of the specificity of ab5514 against EPC1
The siRNA that extinguishes EPC1 antibody-associated signal were independently validated using quantitative RT-PCR.
D: Detection using indirect immunofluorescence of the signal corresponding to EPC1 in 293 cells. Cells were transfected with a control shRNA directed against luciferase (Siluc, left) or two siRNAs directed againstEPC1 (siR1EPC1, middle, and siR2EPC1, right). 24 hours after transfection cells were fixed, blocked, incubated for 1 hr at 37 C with ab5514 at a 1/50 dilution. The cells were then washed three times and incubated for 1 hour at 37 C with a donkey anti-rabbit antibody coupled with Alexa Fluor 488 (Molecular probes) at a 1/500 dilution, washed three times, stained with DAPI and mounted with Vectasheild(Abcyss). E: Efficiency o
All lanes : Anti-EPC1 antibody (ab5514) at 2 µg/ml
Lane 1 : Whole cell lysate from HEK293 cell line
Lane 2 : Whole cell lysate from HeLa cell line
Lane 3 : Whole cell lysate from U20S cell line
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated pig anti rabbit Ig
Performed under reducing conditions.
Exposure time: 15 minutes
Detection method: Supersignal West Dura. Note: There are 3 characterised isoforms of EPC1 which give rise to proteins of 93 kDa, 90 kDa and 85 kDa (Swissprot). This antibody detects a species of ~85 kDa in all lysates, including HEK293 cells which are known to express EPC1. The clean blots, together with the IF data (see datasheet), suggests that this antibody is specific to EPC1 and will work in western blotting.
ab5514 has not yet been referenced specifically in any publications.