Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)
![Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)](https://www.abcam.com/ps/products/216/ab216136/Images/ab216136-411691-anti-epcam-antibody-flow-cytometry-mcf7-human.jpg "Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)")
Key features and details
- Mouse monoclonal [Cocktail EGP40/826/837/1110/1120] to EpCAM
- Suitable for: IHC-P, WB, Flow Cyt
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120]
See all EpCAM primary antibodies -
Description
Mouse monoclonal [Cocktail EGP40/826/837/1110/1120] to EpCAM -
Host species
Mouse -
Specificity
This antibody recognizes extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (Ep-CAM). -
Tested applications
Suitable for: IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rhesus monkey
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Immunogen
Recombinant full length protein corresponding to Human EpCAM aa 1-314.
Sequence:MAPPQVLAFG LLLAAATATF AAAQEECVCE NYKLAVNCFV NNNRQCQCTS VGAQNTVICS KLAAKCLVMK AEMNGSKLGR RAKPEGALQN NDGLYDPDCD ESGLFKAKQC NGTSMCWCVN TAGVRRTDKD TEITCSERVR TYWIIIELKH KAREKPYDSK SLRTALQKEI TTRYQLDPKF ITSILYENNV ITIDLVQNSS QKTQNDVDIA DVAYYFEKDV KGESLFHSKK MDLTVNGEQL DLDPGQTLIY YVDEKAPEFS MQGLKAGVIA VIVVVVIAVV AGIVVLVISR KKRMAKYEKA EIKEMGEMHR ELNA
Database link: P16422 -
Positive control
- IHC-P: Human breast carcinoma tissue. WB: A431 and MCF7 cell lysates. Flow Cyt: MCF7 cells.
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General notes
It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
Cocktail EGP40/826/837/1110/1120 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab216136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 35 kDa. | |
| Flow Cyt | Use 1-2µg for 106 cells. |
Target
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Function
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. -
Tissue specificity
Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. -
Involvement in disease
Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM. -
Sequence similarities
Belongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain. -
Post-translational
modificationsHyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. -
Cellular localization
Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction. - Information by UniProt
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Database links
- Entrez Gene: 4072 Human
- Entrez Gene: 677680 Rhesus monkey
- Omim: 185535 Human
- SwissProt: P16422 Human
- SwissProt: Q1WER1 Rhesus monkey
- Unigene: 542050 Human
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Alternative names
- 17 1A antibody
- 323/A3 antibody
- Adenocarcinoma associated antigen antibody
see all
Images
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Flow Cytometry - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)
Overlay histogram showing MCF-7 (Human breast adenocarcinoma) cells stained with ab216136 (blue) or isotype control (red). The cells were fixed with paraformaldehyde and incubated with ab216136 at 1 ug/million cells.
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![Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)](https://www.abcam.com/ps/products/216/ab216136/Images/ab216136-411663-anti-epcam-antibody-egp4082683711101120-western-blot-a431-wt-a431-epcam-knockout-mcf7-hela.jpg)
Western blot - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)All lanes : Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136) at 0.5 µg/ml
Lane 1 : Wild-type A431 cell lysate
Lane 2 : EPCAM knockout A431 cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab216136 observed at 40 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab216136 was shown to react with EpCAM in wild-type A431 cells in western blot with loss of signal observed in EPCAM knockout sample. Wild-type and EPCAM knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab216136 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 0.5 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)](https://www.abcam.com/ps/products/216/ab216136/Images/ab216136-269341-anti-epcam-antibody-egp40-826-837-1110-1120-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EpCAM antibody [Cocktail EGP40/826/837/1110/1120] (ab216136)Immunohistochemical analysis of paraffin embedded, formalin fixed human breast carcinoma tissue labeling EpCAM with ab216136 at 1 μg/mL.
Protocols
References (0)
ab216136 has not yet been referenced specifically in any publications.
