Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-EpCAM antibody [EPR20533-63]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (gut)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9
Permeabilization
No
Specification
gut
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: goat Serum
Dilution
1/1000

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
H2O2 / 10 min / 22° Streptavidin ABC system (30 min / 22°C) DAB map reagent (10 min / 22°C) Counterstained with Haematoxylin

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Submitted May 28 2019

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