• Product name

    Anti-EpCAM antibody [EPR677(2)]
    See all EpCAM primary antibodies
  • Description

    Rabbit monoclonal [EPR677(2)] to EpCAM
  • Host species

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human EpCAM aa 50-150. The exact sequence is proprietary.

  • Positive control

    • WB: HCT116, A431 and HT29 cell lysates. IHC-P: Human colon adenocarcinoma, colon, endometrial adenocarcinoma and stomach adenocarcinoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab124825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 35 kDa). Western blots of mouse and rat samples are negative.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
    • Tissue specificity

      Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
    • Involvement in disease

      Defects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
      Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
    • Sequence similarities

      Belongs to the EPCAM family.
      Contains 1 thyroglobulin type-1 domain.
    • Post-translational

      Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
    • Cellular localization

      Lateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
    • Information by UniProt
    • Database links

    • Alternative names

      • 17 1A antibody
      • 323/A3 antibody
      • Adenocarcinoma associated antigen antibody
      • Adenocarcinoma-associated antigen antibody
      • Antigen identified by monoclonal antibody AUA1 antibody
      • AUA1 antibody
      • CD326 antibody
      • CD326 antigen antibody
      • Cell surface glycoprotein Trop 1 antibody
      • Cell surface glycoprotein Trop 2 antibody
      • Cell surface glycoprotein Trop-1 antibody
      • CO 17A antibody
      • CO17 1A antibody
      • CO17A antibody
      • DIAR5 antibody
      • EGP 2 antibody
      • EGP antibody
      • EGP2 antibody
      • EGP314 antibody
      • EGP40 antibody
      • Ep CAM antibody
      • Ep-CAM antibody
      • EPCAM antibody
      • EPCAM_HUMAN antibody
      • EpCAM1 antibody
      • Epithelial cell adhesion molecule antibody
      • Epithelial Cell Adhesion Molecule Intracellular Domain (EpCAM-ICD) antibody
      • Epithelial cell surface antigen antibody
      • Epithelial cellular adhesion molecule antibody
      • Epithelial glycoprotein 1 antibody
      • Epithelial glycoprotein 314 antibody
      • Epithelial glycoprotein antibody
      • ESA antibody
      • GA733 1 antibody
      • GA733 2 antibody
      • GA733-2 antibody
      • gastrointestinal tumor-associated antigen 2, 35-KD glycoprotein antibody
      • gp4 antibody
      • hEGP 2 antibody
      • hEGP314 antibody
      • HNPCC8 antibody
      • Human epithelial glycoprotein 2 antibody
      • KS 1/4 antigen antibody
      • KS1/4 antibody
      • KSA antibody
      • Ly74 antibody
      • Lymphocyte antigen 74 antibody
      • M1S 1 antibody
      • M1S2 antibody
      • M4S1 antibody
      • Major gastrointestinal tumor associated protein GA733 2 antibody
      • Major gastrointestinal tumor-associated protein GA733-2 antibody
      • mEGP314 antibody
      • Membrane component chromosome 4 surface marker (35kD glycoprotein) antibody
      • Membrane component, chromosome 4, surface marker 1 antibody
      • Membrane component, chromosome 4, surface marker antibody
      • MIC18 antibody
      • MK 1 antibody
      • Protein 289A antibody
      • TACD1 antibody
      • TACSTD1 antibody
      • TROP1 antibody
      • Tumor associated calcium signal transducer 1 antibody
      • Tumor associated calcium signal transducer 2 precursor antibody
      • Tumor-associated calcium signal transducer 1 antibody
      see all


    • ab124825 showing positive staining in Normal kidney tissue.

    • ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon adenocarcinoma tissue by Immunohistochemistry.
    • All lanes : Anti-EpCAM antibody [EPR677(2)] (ab124825) at 1/1000 dilution

      Lane 1 : HCT116 cell lysate
      Lane 2 : A431 cell lysate
      Lane 3 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 35 kDa

    • ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human colon tissue by Immunohistochemistry.
    • ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human endometrial adenocarcinoma tissue by Immunohistochemistry.
    • ab124825 showing positive staining in Urinary bladder transitional carcinoma tissue.

    • ab124825 showing negative staining in Normal brain tissue.

    • ab124825 showing negative staining in Glioma tissue.

    • ab124825 showing negative staining in Skeletal muscle tissue.

    • ab124825 showing negative staining in Normal tonsil tissue.

    • ab124825, at 1/100 dilution, staining EpCAM in paraffin-embedded Human stomach adenocarcinoma tissue by Immunohistochemistry.


    This product has been referenced in:

    • Natale BV  et al. Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta. Sci Rep 7:5575 (2017). Read more (PubMed: 28717241) »
    • Mori H  et al. Pathobiology of the 129:Stat1 -/- mouse model of human age-related ER-positive breast cancer with an immune infiltrate-excluded phenotype. Breast Cancer Res 19:102 (2017). IHC . Read more (PubMed: 28865492) »
    See all 7 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A


    This EpCAM antibody (3668-1, ab124825) binds an epitope in the extracellular region of the protein.

    Read More
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Cell (Intestine)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

    Frederick Park

    Verified customer

    Submitted Sep 27 2012


    Thank you for contacting us.

    ab124825 and ab71916 are the antibodies as per your requirement.

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