Key features and details
- Mouse monoclonal [VU-1D9] to EpCAM
- Suitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Fr, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-EpCAM antibody [VU-1D9]
See all EpCAM primary antibodies
DescriptionMouse monoclonal [VU-1D9] to EpCAM
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IF, IP, IHC-Fr, IHC-Pmore details
Species reactivityReacts with: Human
Does not react with: Rat
Tissue, cells or virus corresponding to Human EpCAM. Small cell lung carcinoma cells.
- HT29 cells; Human breast tumor; Human colon carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Purification notesPurified by Protein A/G from TCS
Light chain typekappa
- Anti-EpCAM antibody [VU-1D9] (FITC) (ab112067)
- Anti-EpCAM antibody [VU-1D9] (PE) (ab112068)
- Anti-EpCAM antibody [VU-1D9] (PerCP/Cy5.5®) (ab157319)
- Anti-EpCAM antibody [VU-1D9] - BSA and Azide free (ab212579)
- Anti-EpCAM antibody [VU-1D9] (Alexa Fluor® 647) (ab239273)
- Anti-EpCAM antibody [VU-1D9] (APC) (ab239288)
- Anti-EpCAM antibody [VU-1D9] (Biotin) (ab79079)
Our Abpromise guarantee covers the use of ab187372 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 40-43 kDa (predicted molecular weight: 35 kDa).|
|Flow Cyt||Use 0.5-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|IP||Use at 0.5-1 µg/mg of lysate.|
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.|
FunctionMay act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Tissue specificityHighly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Involvement in diseaseDefects in EPCAM are the cause of diarrhea type 5 (DIAR5) [MIM:613217]. It is an intractable diarrhea of infancy characterized by villous atrophy and absence of inflammation, with intestinal epithelial cell dysplasia manifesting as focal epithelial tufts in the duodenum and jejunum.
Defects in EPCAM are a cause of hereditary non-polyposis colorectal cancer type 8 (HNPCC8) [MIM:613244]. HNPCC is a disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early-onset colorectal carcinoma (CRC) and extra-colonic tumors of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world. Clinically, HNPCC is often divided into two subgroups. Type I is characterized by hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II is characterized by increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected. Note=HNPCC8 results from heterozygous deletion of 3-prime exons of EPCAM and intergenic regions directly upstream of MSH2, resulting in transcriptional read-through and epigenetic silencing of MSH2 in tissues expressing EPCAM.
Sequence similaritiesBelongs to the EPCAM family.
Contains 1 thyroglobulin type-1 domain.
modificationsHyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Cellular localizationLateral cell membrane. Cell junction > tight junction. Co-localizes with CLDN7 at the lateral cell membrane and tight junction.
- Information by UniProt
- 17 1A antibody
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ab187372 has been referenced in 1 publication.
- Parker SG et al. A photoelectrochemical platform for the capture and release of rare single cells. Nat Commun 9:2288 (2018). PubMed: 29895867