Anti-EPCR/CD201 antibody [OTI12H5] (ab236517)
Key features and details
- Mouse monoclonal [OTI12H5] to EPCR/CD201
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-EPCR/CD201 antibody [OTI12H5]
See all EPCR/CD201 primary antibodies -
Description
Mouse monoclonal [OTI12H5] to EPCR/CD201 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human EPCR/CD201 aa 1 to the C-terminus. Produced in HEK-293T cells (NP_006395).
Database link: Q9UNN8 -
Positive control
- WB: pCMV6-ENTRY EPCR/CD201 cDNA transfected HEK-293T cell lysate. IHC-P: Human breast, colon, colon adenocarcinoma, lung, endometrium, prostate and bladder tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
OTI12H5 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 27 kDa.
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IHC-P |
1/150.
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes |
Notes |
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WB
1/2000. Predicted molecular weight: 27 kDa. |
IHC-P
1/150. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes |
Target
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Function
Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation. -
Tissue specificity
Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney. -
Post-translational
modificationsN-glycosylated.
A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10544 Human
- Omim: 600646 Human
- SwissProt: Q9UNN8 Human
- Unigene: 647450 Human
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Alternative names
- Activated protein C receptor antibody
- APC receptor antibody
- APCR antibody
see all
Images
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All lanes : Anti-EPCR/CD201 antibody [OTI12H5] (ab236517) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY EPCR/CD201 cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa -
Paraffin-embedded human breast tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon adenocarcinoma tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human lung tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human bladder tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236517 has not yet been referenced specifically in any publications.