Key features and details
- Mouse monoclonal [OTI12H5] to EPCR/CD201
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-EPCR/CD201 antibody [OTI12H5]
See all EPCR/CD201 primary antibodies
DescriptionMouse monoclonal [OTI12H5] to EPCR/CD201
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human EPCR/CD201 aa 1-210. Produced in HEK-293T cells (NP_006395).
MLTTLLPILLLSGWAFCSQDASDGLQRLHMLQISYFRDPYHVWYQGNASL GGHLTHVLEGPDTNTTIIQLQPLQEPESWARTQSGLQSYLLQFHGLVRLV HQERTLAFPLTIRCFLGCELPPEGSRAHVFFEVAVNGSSFVSFRPERALW QADTQVTSGVVTFTLQQLNAYNRTRYELREFLEDTCVQYVQKHISAENTK GSQTSRSYTS
Database link: Q9UNN8
- WB: pCMV6-ENTRY EPCR/CD201 cDNA transfected HEK-293T cell lysate. IHC-P: Human breast, colon, colon adenocarcinoma, lung, endometrium, prostate and bladder tissues.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 27 kDa.|
Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes
FunctionBinds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
Tissue specificityExpressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.
- Information by UniProt
- Activated protein C receptor antibody
- APC receptor antibody
- APCR antibody
All lanes : Anti-EPCR/CD201 antibody [OTI12H5] (ab236517) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY EPCR/CD201 cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
Paraffin-embedded human breast tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human colon tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human colon adenocarcinoma tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human lung tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human bladder tissue stained for EPCR/CD201 using ab236517 at 1/150 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236517 has not yet been referenced specifically in any publications.