Overview

  • Product name

    Anti-EPCR/CD201 antibody [RCR-252]
    See all EPCR/CD201 primary antibodies
  • Description

    Rat monoclonal [RCR-252] to EPCR/CD201
  • Host species

    Rat
  • Tested applications

    Suitable for: Inhibition Assay, Flow Cyt, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tail base Human EPCR/CD201-positive RE-1 cells

  • Epitope

    Antibody RCR-252 stains the extracellular domain of human EPCR.
  • Positive control

    • RE-1 (rat 3Y1 fibroblasts stably transfected with human EPCR).
  • General notes


    Ablates APC binding to EPCR/CD201 thus blocking APC-mediated signalling.

Properties

Applications

Our Abpromise guarantee covers the use of ab81712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use a concentration of 10 - 25 µg/ml. Antibody RCR-252 functions as blocking antbody. It ablates APC binding to EPCR and blocks APC-mediated signaling.
Flow Cyt 1/50.

ab18407 - Rat monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

IP Use at an assay dependent concentration.
WB 1/50. Use under non reducing condition.

Target

  • Function

    Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
  • Tissue specificity

    Expressed strongly in the endothelial cells of arteries and veins in heart and lung, less intensely in capillaries in the lung and skin, and not at all in the endothelium of small vessels of the liver and kidney.
  • Post-translational
    modifications

    N-glycosylated.
    A soluble form exists; probably released by a metalloprotease. Seems to have the same activity as the membrane-bound form.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activated protein C receptor antibody
    • APC receptor antibody
    • APCR antibody
    • bA42O4.2 antibody
    • CCCA antibody
    • CCD41 antibody
    • CD201 antibody
    • CD201 antigen antibody
    • cell cycle, centrosome-associated protein antibody
    • Cell cycle, centrosome-associated protein antibody
    • centrocyclin antibody
    • Endothelial cell protein C receptor antibody
    • Endothelial protein C receptor antibody
    • EPCR antibody
    • EPCR_HUMAN antibody
    • MGC23024 antibody
    • PROCR antibody
    • Protein C receptor, endothelial antibody
    see all

Images

  • Detection of EPCR in HUVEC cells. Red and black line represent the isotype control and ab81712 at 20 μg/ml, respectively.

References

This product has been referenced in:

  • Healy LD  et al. Activated protein C inhibits neutrophil extracellular trap formation in vitro and activation in vivo. J Biol Chem 292:8616-8629 (2017). Read more (PubMed: 28408624) »
  • Dömötör E  et al. Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1. Blood 101:4797-801 (2003). Inhibition Assay ; Human . Read more (PubMed: 12586611) »
See all 3 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Answer

Thank you for your enquiry and interest in our products.

As the datasheet indicates this product (ab81712: Anti-EPCR/CD201 antibody) has been tested and characterized in Inhibition Assay, Flow Cyt, IP, WB so we can offer our guarantee for these applications.

As the disclaimer states on the bottom of each of our datasheets: "all our products are for research use only and are not intended for diagnostic or therapeutic use". This means that we do not recommend our antibodies for in vivo studies and experiments or for medical application.

The unit size is 50 µg and we sell this product at a concentration of 0.1 mg/ml.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

Thanks for your email. I'm very sorry that even the replacement antibody still does not appear to be working as expected.

To help improve your results, Iam happy to provide a replacement of any primary antibody, credit or refund. Please let me know the order reference number associated with purchase of one of these antibodies so I can process your request.

Read More

Answer

Thank you for your reply.

While we do not currently have in house testing data using ab81712 in Flow Cytometry on neutrophils, this clone has been used on human neutrophils in the following paper:

http://bloodjournal.hematologylibrary.org/content/102/4/1499.long

If you are unsatisfied with your results, please let me know your original order number and I will be happy to offer you a free of charge replacement antibody (such as ab15604) or a credit or refund.

Read More

Answer

Thank you for contacting us. I am sorry that this antibody is not giving signal in Flow Cytometry on your human samples. Based on your protocol, my only recommendation would be to increase your primary and secondary antibody incubation times to at least 30 minutes, preferably 1 hour for the primary. I hope this will help to improve your results. If not, please let me know your original order or PO number and I will be happy to offer you a free of charge replacement, credit, or refund.

Read More

Answer

Thank you for contacting Abcam.

Below is a suitable secondary to use with ab81712 in immunocytochemistry:

https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-HRP-pre-adsorbed-ab98425.html



However, I would like to mention that since we have not tested ab81712 in immunocytochemistry we cannot guarantee that it would work for you, but if you have not already purchased the antibody, then you could take advantage of our 100% Abreview Testing discount program:

https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount



If you would like to take advantage of the testing discount program or if there is anything else I can help you with, please let me know.

Read More

Question
Answer

Sure, this should be fine too. ab6519 will react specifically with the Fab fragment of rat IgG.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Answer

Thanks for your enquiry.

ab81712 is a rat monoclonal, IgG1. https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rat-IgG-H-L-PE-pre-adsorbed-ab98392.htmlis a goat anti-rat- PE conjugated secondary antibody, catalog number ab98392. It will react with all isotypes of rat IgG.

I hope this is helpful. Please contact me again if you have any further questions.

Read More

Question
Answer

Thank you for contacting Abcam.

For ab81712, I would recommend that you use ab37361 as an isotype control:

https://www.abcam.com/Rat-IgG-Isotype-Control-ab37361.html



If there is anything else I can help you with, please let me know.

Read More

Question
Answer

Thank you for your phone call.

DISCOUNT CODE: xxx
Expiration date: xxx

I am very pleased to hear you would like to accept our offer and test ab81712 in dog and ICC. This code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for dog and ICC, and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thanks for your call yesterday and for your patience while I have been in touch with the lab about ab81712. The production lab did not test this antibody in-house in Western blot, and the Western blot information originates from the paper that I previously sent. According to this article, the immunoprecipitates were run on a 10% reducing gel then immunoblotted, so I think it should be fine to use the antibody against reduced samples. I hope this information is useful, but please let me know if you need anything further and I'll be happy to help.

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up