Overview

  • Product name

  • Description

    Rabbit polyclonal to EPF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human EPF aa 51-100. (NP_002148.1)
    Sequence:

    SGSKGKGGEI QPVSVKVGDK VLLPEYGGTK VVLDDKDYFL FRDGDILGKY


    Database link: P61604

  • Positive control

    • Human prostate tissue; NIH 3T3 cells; NIH 3T3 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab189264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.
WB 1/500 - 1/1000. Predicted molecular weight: 11 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function

    Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
  • Sequence similarities

    Belongs to the GroES chaperonin family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 10 kDa chaperonin antibody
    • 10 kDa heat shock protein mitochondrial antibody
    • 10 kDa heat shock protein, mitochondrial antibody
    • CH10_HUMAN antibody
    • Chaperonin 10 antibody
    • Chaperonin 10 homolog antibody
    • CPN10 antibody
    • cpn10 homolog antibody
    • Early pregnancy factor antibody
    • Early-pregnancy factor antibody
    • EPF antibody
    • GROES antibody
    • GroES homolog antibody
    • Heat shock 10kD protein 1 chaperonin 10 antibody
    • Heat shock 10kDa protein 1 antibody
    • Heat shock 10kDa protein 1 chaperonin 10 antibody
    • Heat shock protein family E (Hsp10) member antibody
    • Heat-shock 10-kD protein antibody
    • Hsp10 antibody
    • Hspe1 antibody
    see all

Images

  • All lanes : Anti-EPF antibody (ab189264) at 1/500 dilution

    Lane 1 : NIH3T3 cell lysate with Without immunizing peptide
    Lane 2 : NIH3T3 cell lysate with With immunizing peptide

    Predicted band size: 11 kDa

  • Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human prostate tissue labeling EPF using ab189264 at 10 µg/ml.

  • Immunofluorescence of NIH-3T3 cells, labeling MPF using ab189264 at 1/100 dilution. The picture on the right is treated with the synthesized peptide.

References

ab189264 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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