Key features and details
- Rabbit polyclonal to EPF
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-EPF antibody
See all EPF primary antibodies
DescriptionRabbit polyclonal to EPF
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Cow, Human
- WB: Rat brain lysate. IHC: Mouse back skin tissue.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab223358 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use Bouin’s Fixative Solution.
|WB||1/1000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).|
FunctionEukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
Sequence similaritiesBelongs to the GroES chaperonin family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
Mouse back skin stained for EPF with ab223358 at 1/100 dilution in immunohistochemical analysis, followed by Goat Anti-Rabbit FITC at 1/50 dilution for 1 hour at room temperature. Fixation: Bouin’s Fixative Solution.
Anti-EPF antibody (ab223358) at 1/1000 dilution + Rat brain lysate at 15 µg
Donkey Anti-Rabbit IgG HRP
Predicted band size: 10 kDa
Observed band size: 10 kDa
Blocking buffer: 1.5 % BSA.
Primary incubation: 2 hours at room temperature.
ab223358 has not yet been referenced specifically in any publications.