Anti-EPF antibody (ab227428)
Key features and details
- Rabbit polyclonal to EPF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-EPF antibody
See all EPF primary antibodies -
Description
Rabbit polyclonal to EPF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human EPF.
Database link: P61604 -
Positive control
- WB: Mouse brain lysate. IHC: Human lung carcinoma tissue. ICC/IF: HeLa, U-2 OS and SK-N-SH cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 11 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 11 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. -
Sequence similarities
Belongs to the GroES chaperonin family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 281833 Cow
- Entrez Gene: 3336 Human
- Entrez Gene: 15528 Mouse
- Entrez Gene: 397575 Pig
- Entrez Gene: 25462 Rat
- Omim: 600141 Human
- SwissProt: P61603 Cow
- SwissProt: P61604 Human
see all -
Alternative names
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
see all
Images
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Anti-EPF antibody (ab227428) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa15% SDS PAGE
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EPF (green) using ab227428 at 1/100 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstained: alpha tubulin (red) and Hoechst 33342 (blue).
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Paraffin-embedded human lung carcinoma tissue stained for EPF using ab227428 at 1/250 dilution in immunohistochemical analysis.
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U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EPF (green) using ab227428 at 1/200 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstained: alpha tubulin (red) and Hoechst 33342 (blue).
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SK-N-SH (human neuroblastoma cell line) cells stained for EPF (green) using ab227428 at 1/500 dilution in ICC/IF. Cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 15 minutes. Counterstained : Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227428 has not yet been referenced specifically in any publications.