Overview

  • Product name

  • Description

    Rabbit polyclonal to EPF
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human EPF.
    Database link: P61604

  • Positive control

    • WB: Mouse brain lysate. IHC: Human lung carcinoma tissue. ICC/IF: HeLa, U-2 OS and SK-N-SH cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 11 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
  • Sequence similarities

    Belongs to the GroES chaperonin family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 10 kDa chaperonin antibody
    • 10 kDa heat shock protein mitochondrial antibody
    • 10 kDa heat shock protein, mitochondrial antibody
    • CH10_HUMAN antibody
    • Chaperonin 10 antibody
    • Chaperonin 10 homolog antibody
    • CPN10 antibody
    • cpn10 homolog antibody
    • Early pregnancy factor antibody
    • Early-pregnancy factor antibody
    • EPF antibody
    • GROES antibody
    • GroES homolog antibody
    • Heat shock 10kD protein 1 chaperonin 10 antibody
    • Heat shock 10kDa protein 1 antibody
    • Heat shock 10kDa protein 1 chaperonin 10 antibody
    • Heat shock protein family E (Hsp10) member antibody
    • Heat-shock 10-kD protein antibody
    • Hsp10 antibody
    • Hspe1 antibody
    see all

Images

  • Anti-EPF antibody (ab227428) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 11 kDa



    15% SDS PAGE

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EPF (green) using ab227428 at 1/100 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstained: alpha tubulin (red) and Hoechst 33342 (blue).

  • Paraffin-embedded human lung carcinoma tissue stained for EPF using ab227428 at 1/250 dilution in immunohistochemical analysis.

  • U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EPF (green) using ab227428 at 1/200 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstained: alpha tubulin (red) and Hoechst 33342 (blue).

  • SK-N-SH (human neuroblastoma cell line) cells stained for EPF (green) using ab227428 at 1/500 dilution in ICC/IF. Cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 15 minutes. Counterstained : Hoechst 33342 (blue).

References

ab227428 has not yet been referenced specifically in any publications.

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