Key features and details
- Rabbit polyclonal to EPF
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-EPF antibody
See all EPF primary antibodies
DescriptionRabbit polyclonal to EPF
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Rhesus monkey
Recombinant full length protein corresponding to Human EPF.
Database link: P61604
- WB: Mouse brain lysate. IHC: Human lung carcinoma tissue. ICC/IF: HeLa, U-2 OS and SK-N-SH cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 11 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionEukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
Sequence similaritiesBelongs to the GroES chaperonin family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
Anti-EPF antibody (ab227428) at 1/1000 dilution + Mouse brain tissue lysate at 50 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
15% SDS PAGE
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for EPF (green) using ab227428 at 1/100 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstained: alpha tubulin (red) and Hoechst 33342 (blue).
Paraffin-embedded human lung carcinoma tissue stained for EPF using ab227428 at 1/250 dilution in immunohistochemical analysis.
U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for EPF (green) using ab227428 at 1/200 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes. Counterstained: alpha tubulin (red) and Hoechst 33342 (blue).
SK-N-SH (human neuroblastoma cell line) cells stained for EPF (green) using ab227428 at 1/500 dilution in ICC/IF. Cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 15 minutes. Counterstained : Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227428 has not yet been referenced specifically in any publications.