Recombinant Anti-EPF antibody [EPR4475] (ab108611)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4475] to EPF
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-EPF antibody [EPR4475]
See all EPF primary antibodies -
Description
Rabbit monoclonal [EPR4475] to EPF -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, HepG2, Raji, and Jurkat cell lysates. Permeabilized Jurkat cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4475 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab108611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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WB |
Human
|
All applications |
Rat
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
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|
Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). |
Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. -
Sequence similarities
Belongs to the GroES chaperonin family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3336 Human
- Entrez Gene: 25462 Rat
- Omim: 600141 Human
- SwissProt: P61604 Human
- SwissProt: P26772 Rat
- Unigene: 1197 Human
- Unigene: 106093 Rat
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Alternative names
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
see all
Images
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All lanes : Anti-EPF antibody [EPR4475] (ab108611) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa -
Flow cytometric analysis of permeabilized Jurkat cells using ab108611 at 1/10 (red) or a Rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab108611 has been referenced in 1 publication.
- Kovács ÁF et al. Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation. Int J Mol Sci 20:N/A (2019). PubMed: 31337116