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Signal Transduction Protein Trafficking Chaperones Heat Shock Proteins
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RecombinantRabMAb

Recombinant Anti-EPF antibody [EPR4475] (ab108611)

  • Datasheet
  • SDS
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Product price, shipping and contact information

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Western blot - Anti-EPF antibody [EPR4475] (ab108611)
  • Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)
  • Anti-EPF antibody [EPR4475] (ab108611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4475] to EPF
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Recombinant E. coli EPF protein (ab51308)

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Overview

  • Product name

    Anti-EPF antibody [EPR4475]
    See all EPF primary antibodies
  • Description

    Rabbit monoclonal [EPR4475] to EPF
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, Raji, and Jurkat cell lysates. Permeabilized Jurkat cells.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4475
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-EPF antibody [EPR4475] - BSA and Azide free (ab244230)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant E. coli EPF protein (ab51308)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Notes
WB
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.
    • Sequence similarities

      Belongs to the GroES chaperonin family.
    • Cellular localization

      Mitochondrion matrix.
    • Target information above from: UniProt accession P61604 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 3336 Human
      • Entrez Gene: 25462 Rat
      • Omim: 600141 Human
      • SwissProt: P61604 Human
      • SwissProt: P26772 Rat
      • Unigene: 1197 Human
      • Unigene: 106093 Rat
      • Alternative names

        • 10 kDa chaperonin antibody
        • 10 kDa heat shock protein mitochondrial antibody
        • 10 kDa heat shock protein, mitochondrial antibody
        • CH10_HUMAN antibody
        • Chaperonin 10 antibody
        • Chaperonin 10 homolog antibody
        • CPN10 antibody
        • cpn10 homolog antibody
        • Early pregnancy factor antibody
        • Early-pregnancy factor antibody
        • EPF antibody
        • GROES antibody
        • GroES homolog antibody
        • Heat shock 10kD protein 1 chaperonin 10 antibody
        • Heat shock 10kDa protein 1 antibody
        • Heat shock 10kDa protein 1 chaperonin 10 antibody
        • Heat shock protein family E (Hsp10) member antibody
        • Heat-shock 10-kD protein antibody
        • Hsp10 antibody
        • Hspe1 antibody
        see all

      Images

      • Western blot - Anti-EPF antibody [EPR4475] (ab108611)
        Western blot - Anti-EPF antibody [EPR4475] (ab108611)
        All lanes : Anti-EPF antibody [EPR4475] (ab108611) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 11 kDa
        Observed band size: 11 kDa

      • Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)
        Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)
        Flow cytometric analysis of permeabilized Jurkat cells using ab108611 at 1/10 (red) or a Rabbit IgG (negative) (green).
      • Anti-EPF antibody [EPR4475] (ab108611)
        Anti-EPF antibody [EPR4475] (ab108611)

      Protocols

      • Western blot protocols
      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab108611? Please let us know so that we can cite the reference in this datasheet.

      ab108611 has been referenced in 1 publication.

      • Kovács ÁF  et al. Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation. Int J Mol Sci 20:N/A (2019). PubMed: 31337116

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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