RecombinantRabMAb

Recombinant Anti-EPF antibody [EPR4475] (ab108611)

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Western blot - Anti-EPF antibody [EPR4475] (ab108611)
  • Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)
  • Anti-EPF antibody [EPR4475] (ab108611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4475] to EPF
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-EPF antibody [EPR4475]
    See all EPF primary antibodies

  • Description

    Rabbit monoclonal [EPR4475] to EPF

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, HepG2, Raji, and Jurkat cell lysates. Permeabilized Jurkat cells.

  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
WB
Human
All applications
Rat
Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa).
Flow Cyt
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC or IHC-P.

    • Target

    • Function

      Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter.

    • Sequence similarities

      Belongs to the GroES chaperonin family.

    • Cellular localization

      Mitochondrion matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • 10 kDa chaperonin antibody
        • 10 kDa heat shock protein mitochondrial antibody
        • 10 kDa heat shock protein, mitochondrial antibody
        • CH10_HUMAN antibody
        • Chaperonin 10 antibody
        • Chaperonin 10 homolog antibody
        • CPN10 antibody
        • cpn10 homolog antibody
        • Early pregnancy factor antibody
        • Early-pregnancy factor antibody
        • EPF antibody
        • GROES antibody
        • GroES homolog antibody
        • Heat shock 10kD protein 1 chaperonin 10 antibody
        • Heat shock 10kDa protein 1 antibody
        • Heat shock 10kDa protein 1 chaperonin 10 antibody
        • Heat shock protein family E (Hsp10) member antibody
        • Heat-shock 10-kD protein antibody
        • Hsp10 antibody
        • Hspe1 antibody
        see all

      Images

      • Western blot - Anti-EPF antibody [EPR4475] (ab108611)
        Western blot - Anti-EPF antibody [EPR4475] (ab108611)
        All lanes : Anti-EPF antibody [EPR4475] (ab108611) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HepG2 cell lysate
        Lane 3 : Raji cell lysate
        Lane 4 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 11 kDa
        Observed band size: 11 kDa

      • Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)
        Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)
        Flow cytometric analysis of permeabilized Jurkat cells using ab108611 at 1/10 (red) or a Rabbit IgG (negative) (green).
      • Anti-EPF antibody [EPR4475] (ab108611)
        Anti-EPF antibody [EPR4475] (ab108611)

      References (1)

      Publishing research using ab108611? Please let us know so that we can cite the reference in this datasheet.

      ab108611 has been referenced in 1 publication.

      • Kovács ÁF  et al. Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation. Int J Mol Sci 20:N/A (2019). PubMed: 31337116

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