Recombinant Anti-EPF antibody [EPR4475] (ab108611)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-EPF antibody [EPR4475]
See all EPF primary antibodies -
Description
Rabbit monoclonal [EPR4475] to EPF -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide within Human EPF aa 50-150. The exact sequence is proprietary.
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Positive control
- HeLa, HepG2, Raji, and Jurkat cell lysates. Permeabilized Jurkat cells.
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General notes
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4475 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab108611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). | |
| IP | 1/10 - 1/100. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. -
Sequence similarities
Belongs to the GroES chaperonin family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 3336 Human
- Entrez Gene: 25462 Rat
- Omim: 600141 Human
- SwissProt: P61604 Human
- SwissProt: P26772 Rat
- Unigene: 1197 Human
- Unigene: 106093 Rat
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Alternative names
- 10 kDa chaperonin antibody
- 10 kDa heat shock protein mitochondrial antibody
- 10 kDa heat shock protein, mitochondrial antibody
see all
Images
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![Western blot - Anti-EPF antibody [EPR4475] (ab108611)](https://www.abcam.com/ps/products/108/ab108611/Images/ab108611-121338-anti-epf-antibody-epr4475-western-blot.jpg)
Western blot - Anti-EPF antibody [EPR4475] (ab108611)All lanes : Anti-EPF antibody [EPR4475] (ab108611) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
Observed band size: 11 kDa -
![Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)](https://www.abcam.com/ps/products/108/ab108611/Images/ab108611-121337-anti-epf-antibody-epr4475-flow-cytometry.jpg)
Flow Cytometry - Anti-EPF antibody [EPR4475] (ab108611)Flow cytometric analysis of permeabilized Jurkat cells using ab108611 at 1/10 (red) or a Rabbit IgG (negative) (green).
Datasheets and documents
References
This product has been referenced in:
- Kovács ÁF et al. Unravelling the Role of Trophoblastic-Derived Extracellular Vesicles in Regulatory T Cell Differentiation. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 31337116) »
