Western blot abreview for Anti-Cpn10 antibody [EPR4476]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Loading amount
10 µg
Specification
Jurkat
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Blocking buffer

Secondary antibody

Name
Non-Abcam antibody was used: Anti-Rabbit IgG, HRP-Linked Whole Ab Donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 11 kDa

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Submitted May 02 2012

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