Recombinant
RabMAb

Recombinant Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)

Overview

  • Product name

    Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free
    See all EPH A3+A4+A5 primary antibodies
  • Description

    Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4  phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human EPH A3+A4+A5 (phospho Y779 + Y779 + Y833). The exact sequence is proprietary. P29320, P54756, P54764.

  • Positive control

    • HeLa cell lysate (untreated/treated with Sodium Orthovanadate). Prepare 500mM stock Sodium Orthovanadate in PBS. When ready to treat cells add 400µl 500mM Sodium Orthovanadate to 1560µl H2O and then 40µl H2O2, vortex and incubate at RT for 5-10min. Serum-starve cells overnight, and treat with the freshly made Pervanadate for 20min at 37°C.
  • General notes

    Ab188171 is the carrier-free version of ab124881. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab188171 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab188171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 133 kDa (predicted molecular weight: 110, 115 kDa).
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Relevance

      Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal andhippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion.
    • Cellular localization

      Cell Membrane
    • Database links

    • Alternative names

      • CEK7 antibody
      • EHK 1 antibody
      • EHK1 antibody
      • EK4 antibody
      • EK7 antibody
      • EK8 antibody
      • EPH homology kinase 1 antibody
      • EPH like kinase 4 antibody
      • EPH like kinase 7 antibody
      • EPH like kinase 8 antibody
      • Eph like tyrosine kinase 1 antibody
      • EPH receptor A3 antibody
      • EPH receptor A4 antibody
      • EPH receptor A5 antibody
      • EPHA3 antibody
      • EPHA4 antibody
      • EPHA5 antibody
      • Ephrin type A receptor 3 antibody
      • Ephrin type A receptor 4 antibody
      • Ephrin type A receptor 5 antibody
      • ETK antibody
      • ETK1 antibody
      • HEK antibody
      • HEK4 antibody
      • HEK7 antibody
      • HEK8 antibody
      • Human embryo kinase 1 antibody
      • OTTHUMP00000159594 antibody
      • OTTHUMP00000159595 antibody
      • OTTHUMP00000164185 antibody
      • OTTHUMP00000204491 antibody
      • OTTHUMP00000213903 antibody
      • OTTHUMP00000213904 antibody
      • OTTHUMP00000213905 antibody
      • OTTHUMP00000218917 antibody
      • OTTHUMP00000223029 antibody
      • Receptor protein tyrosine kinase HEK7 antibody
      • Receptor protein tyrosine kinase HEK8 antibody
      • SEK antibody
      • TYRO1 antibody
      • TYRO1 protein tyrosine kinase antibody
      • TYRO4 antibody
      • TYRO4 protein tyrosine kinase antibody
      • Tyrosine protein kinase receptor EHK 1 antibody
      • Tyrosine protein kinase receptor ETK1 antibody
      • Tyrosine protein kinase receptor SEK antibody
      • Tyrosine protein kinase TYRO1 antibody
      see all

    References

    ab188171 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab188171.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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