Recombinant Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
![Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)](https://www.abcam.com/ps/products/188/ab188171/Images/ab188171-406283-anti-eph-a3-a4-a5-phospho-y779-y779-y833-antibody-epr5684-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free
See all EPH A3+A4+A5 primary antibodies -
Description
Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4 phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833.
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Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human EPH A3+A4+A5 (phospho Y779 + Y779 + Y833). The exact sequence is proprietary. P29320, P54756, P54764.
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Positive control
- HeLa cell lysate (untreated/treated with Sodium Orthovanadate). Prepare 500mM stock Sodium Orthovanadate in PBS. When ready to treat cells add 400µl 500mM Sodium Orthovanadate to 1560µl H2O and then 40µl H2O2, vortex and incubate at RT for 5-10min. Serum-starve cells overnight, and treat with the freshly made Pervanadate for 20min at 37°C.
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General notes
Ab188171 is the carrier-free version of ab124881. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab188171 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.30 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5684 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab188171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 133 kDa (predicted molecular weight: 110, 115 kDa). |
Target
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Relevance
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal andhippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 2042 Human
- Entrez Gene: 2043 Human
- Entrez Gene: 2044 Human
- Entrez Gene: 13837 Mouse
- Entrez Gene: 13838 Mouse
- Entrez Gene: 13839 Mouse
- Entrez Gene: 29210 Rat
- Entrez Gene: 316539 Rat
see all -
Alternative names
- CEK7 antibody
- EHK 1 antibody
- EHK1 antibody
see all
Images
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Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/10000 dilution (purified)
Lane 1 : Untreated HEK293 cell lysate
Lane 2 : HEK293 cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 110, 115 kDa
Observed band size: 133 kDa why is the actual band size different from the predicted?This data was developed using ab124881, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)](https://www.abcam.com/ps/products/188/ab188171/Images/ab188171-403069-anti-eph-a3-a4-a5-phospho-y779-y779-y833-antibody-epr5684-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110, 115 kDaThis data was developed using ab124881, the same antibody clone in a different buffer formulation.
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![OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)](https://www.abcam.com/ps/products/188/ab188171/Images/ab188171-1-anti-eph-a3-a4-a5-phospho-y779-y779-y833-antibody-epr5684-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)This data was developed using ab124881, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD -
![Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)](https://www.abcam.com/ps/products/188/ab188171/Images/ab188171-4-benefits-of-recombinant-antibodies.png)
Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] - BSA and Azide free (ab188171)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab188171 has not yet been referenced specifically in any publications.
