Recombinant Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684]
See all EPH A3+A4+A5 primary antibodies -
Description
Rabbit monoclonal [EPR5684] to EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) -
Host species
Rabbit -
Specificity
This antibody only detects EPH A3 phosphorylated at Tyrosine 779, EPH A4 phosphorylated at Tyrosine 779, and EPH A5 phosphorylated at Tyrosine 833. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human EPH A3+A4+A5 (phospho Y779 + Y779 + Y833). The exact sequence is proprietary. P29320, P54756, P54764.
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Positive control
- HeLa whole cell lysate (ab150035) (untreated/treated with Sodium Orthovanadate). Prepare 500mM stock Sodium Orthovanadate in PBS. When ready to treat cells add 400µl 500mM Sodium Orthovanadate to 1560µl H2O and then 40µl H2O2, vortex and incubate at RT for 5-10min. Serum-starve cells overnight, and treat with the freshly made Pervanadate for 20min at 37°C.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.30 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5684 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 110, 115 kDa).
For unpurified, use 1/1000 - 1/10000. |
Notes |
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WB
1/10000. Detects a band of approximately 133 kDa (predicted molecular weight: 110, 115 kDa). For unpurified, use 1/1000 - 1/10000. |
Target
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Relevance
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal andhippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. -
Cellular localization
Cell Membrane -
Database links
- Entrez Gene: 2042 Human
- Entrez Gene: 2043 Human
- Entrez Gene: 2044 Human
- Entrez Gene: 13837 Mouse
- Entrez Gene: 13838 Mouse
- Entrez Gene: 13839 Mouse
- Entrez Gene: 29210 Rat
- Entrez Gene: 316539 Rat
see all -
Alternative names
- CEK7 antibody
- EHK 1 antibody
- EHK1 antibody
see all
Images
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All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/10000 dilution (purified)
Lane 1 : Untreated HEK293 cell lysate
Lane 2 : HEK293 cell lysate treated with pervanadate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 110, 115 kDa
Observed band size: 133 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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All lanes : Anti-EPH A3+A4+A5 (phospho Y779 + Y779 + Y833) antibody [EPR5684] (ab124881) at 1/1000 dilution (unpurified)
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Sodium Orthovanadate. See suggested positive control for more treatment information.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 110, 115 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124881 has been referenced in 1 publication.
- Yu Y et al. Protein Tyrosine Phosphatase Receptor Type J (PTPRJ) Regulates Retinal Axonal Projections by Inhibiting Eph and Abl Kinases in Mice. J Neurosci 38:8345-8363 (2018). PubMed: 30082414