Anti-Eph receptor A1/EphA1 antibody (ab217363)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Eph receptor A1/EphA1 antibody -
Description
Rabbit polyclonal to Eph receptor A1/EphA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Eph receptor A1/EphA1 aa 140-182 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Sequence:CLRLKVTVSGKITHSPQAHDNPQEKRLAADDPEVRVLHSIGHS
Database link: P20827-1 -
Positive control
- IHC-P: Human lung carcinoma tissue.
-
General notes
Previously labelled as Eph receptor A1.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
Our Abpromise guarantee covers the use of ab217363 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. |
Target
-
Function
Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis. -
Tissue specificity
Overexpressed in several carcinomas. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsPhosphorylated. Autophosphorylation is stimulated by its ligand EFNA1.
Ubiquitinated. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2041 Human
- Omim: 179610 Human
- SwissProt: P21709 Human
- Unigene: 89839 Human
-
Alternative names
- EPH antibody
- EPH receptor A1 antibody
- EPH tyrosine kinase 1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A1/EphA1 antibody (ab217363)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue labeling Ephrin A1 with ab217363 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
References
ab217363 has not yet been referenced specifically in any publications.
