Overview

  • Product name

    Anti-Eph receptor A2 antibody
    See all Eph receptor A2 primary antibodies
  • Description

    Rabbit polyclonal to Eph receptor A2
  • Host species

    Rabbit
  • Specificity

    The antibody detects endogenous levels of total Eph receptor A2 protein.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Eph receptor A2. Synthesized non-phosphopeptide derived from human EPHA2 around the phosphorylation site of tyrosine 594. Swiss-Prot: P29317/ P54756.

  • Positive control

    • Extracts from Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 108 kDa.

Target

  • Function

    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.
  • Tissue specificity

    Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
  • Involvement in disease

    Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications

    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARCC2 antibody
    • AW545284 antibody
    • CTPA antibody
    • CTPP1 antibody
    • CTRCT6 antibody
    • EC 2.7.10.1 antibody
    • Eck antibody
    • Eph receptor A2 antibody
    • EPHA2 antibody
    • EPHA2_HUMAN antibody
    • Ephrin receptor antibody
    • Ephrin receptor EphA2 antibody
    • Ephrin type A receptor 2 antibody
    • Ephrin type-A receptor 2 antibody
    • Epithelial cell kinase antibody
    • Epithelial cell receptor protein tyrosine kinase antibody
    • Myk 2 antibody
    • Myk2 antibody
    • Sek 2 antibody
    • Sek2 antibody
    • Soluble EPHA2 variant 1 antibody
    • Tyrosine protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor MPK-5 antibody
    • Tyrosine-protein kinase receptor SEK-2 antibody
    see all

Images

  • All lanes : Anti-Eph receptor A2 antibody (ab196887) at 1/500 dilution

    Lane 1 : Extracts from JK cells treated with the synthesized peptide.
    Lane 2 : Extracts from JK cells

    Predicted band size: 108 kDa

References

ab196887 has not yet been referenced specifically in any publications.

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