Overview

  • Product name

    Anti-Eph receptor A2 antibody [EP4760]
    See all Eph receptor A2 primary antibodies
  • Description

    Rabbit monoclonal [EP4760] to Eph receptor A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Eph receptor A2 aa 50-150 (extracellular). The exact sequence is proprietary.

  • Positive control

    • A431, 293T, and BXPC-3 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab133501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.
    • Tissue specificity

      Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
    • Involvement in disease

      Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
      Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Post-translational
      modifications

      Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • ARCC2 antibody
      • AW545284 antibody
      • CTPA antibody
      • CTPP1 antibody
      • CTRCT6 antibody
      • EC 2.7.10.1 antibody
      • Eck antibody
      • Eph receptor A2 antibody
      • EPHA2 antibody
      • EPHA2_HUMAN antibody
      • Ephrin receptor antibody
      • Ephrin receptor EphA2 antibody
      • Ephrin type A receptor 2 antibody
      • Ephrin type-A receptor 2 antibody
      • Epithelial cell kinase antibody
      • Epithelial cell receptor protein tyrosine kinase antibody
      • Myk 2 antibody
      • Myk2 antibody
      • Sek 2 antibody
      • Sek2 antibody
      • Soluble EPHA2 variant 1 antibody
      • Tyrosine protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor ECK antibody
      • Tyrosine-protein kinase receptor MPK-5 antibody
      • Tyrosine-protein kinase receptor SEK-2 antibody
      see all

    Images

    • All lanes : Anti-Eph receptor A2 antibody [EP4760] (ab133501) at 1/10000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : BXPC-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 108 kDa
      Observed band size: 130 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133501 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133501.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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