Gel Running Conditions
Non-reduced Denaturing (10% gel)
Mouse Cell lysate - whole cell (mouse embryonic stem cell)
mouse embryonic stem cell
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk (blocking buffer)
Abcam antibody:Goat Anti-Rat IgG H&L (HRP)
10 minute(s) and 0 second(s)
Mouse embryonic stem cells in lanes 1-4. Lanes 1 and 2 are reduced, lanes 3 and 4 and non-reduced samples. 5% BSA block was also tried, but did not improve the result.
Supersensitive detection was also used, but only brought up multiple (20+) non specific bands in every lane.
Thank you for sharing this data with us. ab73254 has not previously been tested in western blot and based on this abreview, this antibody is not suitable for this application, under these experimental conditions. Abcam would welcome feedback from other customers using this product.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Submitted Mar 11 2014