Application
Immunocytochemistry/ Immunofluorescence
Blocking step
1% serum, 0.1% BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C
Sample
Mouse Cell (embryonic stem cell)
Specification
embryonic stem cell
Permeabilization
Yes - 0.1% triton X
Fixative
methanol:acetone
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: blocking buffer
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rat Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
WT ES cell staining is shown. I also stained a mutant cell line known to have reduced Ephrin A2 expression and correspondingly reduced staining was seen. PFA fixation was also tried but did not work anywhere near as well as methanol:acetone.
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Submitted Apr 04 2014