Recombinant
RabMAb

Recombinant Anti-Eph receptor A2 antibody [SP169] (ab150304)

Overview

  • Product name

    Anti-Eph receptor A2 antibody [SP169]
    See all Eph receptor A2 primary antibodies
  • Description

    Rabbit monoclonal [SP169] to Eph receptor A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Monkey
  • Immunogen

    Synthetic peptide within Human Eph receptor A2 aa 350-450 (internal sequence). The exact sequence is proprietary.
    Database link: P29317

  • Positive control

    • IHC-P: Human breast carcinoma tissue. Flow Cyt: A431 cells.
  • General notes

     

     

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/100. Boil tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min.

Target

  • Function

    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A3, -A4 and -A5. Plays an important role in angiogenesis and tumor neovascularization. The recruitement of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By similarity). Induces apoptosis in a p53/TP53-independent, caspase-8-dependent manner.
  • Tissue specificity

    Expressed in brain and glioma tissue and glioma cell lines (at protein level). Expressed most highly in tissues that contain a high proportion of epithelial cells, e.g., skin, intestine, lung, and ovary.
  • Involvement in disease

    Genetic variations in EPHA2 are the cause of susceptibility to cataract cortical age-related type 2 (ARCC2) [MIM:613020]. A developmental punctate opacity common in the cortex and present in most lenses. The cataract is white or cerulean, increases in number with age, but rarely affects vision.
    Defects in EPHA2 are the cause of cataract posterior polar type 1 (CTPP1) [MIM:116600]. A subcapsular opacity, usually disk-shaped, located at the back of the lens. It can have a marked effect on visual acuity.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications

    Activated by EFNA1 via tyrosine phosphorylation. Phosphorylated residues Tyr-588 and Tyr-594 are required for binding VAV2 and VAV3 while phosphorylated residues Tyr-735 and Tyr-930 are required for binding PI3-kinase p85 subunit. These phosphorylated residues are critical for recruitment of VAV2 and VAV3 and PI3-kinase p85 subunit which transduce downstream signaling to activate RAC1 GTPase and endothelial cell migration. They also play a critical role in transducing EPHA2 signaling in vascular endothelial cells during tumor angiogenesis.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARCC2 antibody
    • AW545284 antibody
    • CTPA antibody
    • CTPP1 antibody
    • CTRCT6 antibody
    • EC 2.7.10.1 antibody
    • Eck antibody
    • Eph receptor A2 antibody
    • EPHA2 antibody
    • EPHA2_HUMAN antibody
    • Ephrin receptor antibody
    • Ephrin receptor EphA2 antibody
    • Ephrin type A receptor 2 antibody
    • Ephrin type-A receptor 2 antibody
    • Epithelial cell kinase antibody
    • Epithelial cell receptor protein tyrosine kinase antibody
    • Myk 2 antibody
    • Myk2 antibody
    • Sek 2 antibody
    • Sek2 antibody
    • Soluble EPHA2 variant 1 antibody
    • Tyrosine protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor ECK antibody
    • Tyrosine-protein kinase receptor MPK-5 antibody
    • Tyrosine-protein kinase receptor SEK-2 antibody
    see all

Images

  • Flow cytometric analysis of rabbit anti-Eph receptor A2 (SP169) antibody ab150304 (1/100) in A431 (Human epidermoid carcinoma cell line) cells (green) compared to negative control of rabbit IgG (blue).

  • Immunohistochemical analysis of formalin fixed, paraffin embedded human breast carcinoma tissue labeling Eph receptor A2 with ab150304 at 1/100 dilution.

References

This product has been referenced in:

  • Macy ME  et al. A pediatric trial of radiation/cetuximab followed by irinotecan/cetuximab in newly diagnosed diffuse pontine gliomas and high-grade astrocytomas: A Pediatric Oncology Experimental Therapeutics Investigators' Consortium study. Pediatr Blood Cancer 64:N/A (2017). Read more (PubMed: 28544128) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Protein block serum-free as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH 8
Sample
Human Tissue sections (Breast cancer)
Specification
Breast cancer
Permeabilization
No
Fixative
Formalin

Abcam user community

Verified customer

Submitted Nov 27 2013

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