Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody (ab62256)

Overview

  • Product name
    Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody
    See all Eph receptor A2+A3+A4 primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor A2+A3+A4 (phospho Y588 + Y596)
  • Host species
    Rabbit
  • Specificity
    ab62256 detects endogenous levels of Eph receptor A2+A3+A4 only when phosphorylated at tyrosine 588/596.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Sea urchin
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Eph receptor A2+A3+A4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

Images

  • ab62256 at 1/500 - 1/1000 dilution staining Eph receptor A2+A3+A4 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References

This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Sea Urchin Cell (Embryo, Strongylocentrotus purpuratus)
Specification
Embryo, Strongylocentrotus purpuratus
Fixative
Paraformaldehyde
Permeabilization
Yes - Superblock with 0.03% TritonX-100, 1 mM Na3VO4, 1 mM NaF
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 20°C

Dr. Robert Burke

Verified customer

Submitted Mar 28 2013

Answer

Thank you for contacting us.

The lab sent me more information about the immunogen of the antibody ab62256:

The antibody is against both EPHA2 and EPHA3. This antibody recognizes EPHA2 when it is phosphorylated at Y588 and EPHA3 when it is phosphorylated at Y596. Also, alignment of our immunogen sequence with the provided sea urchin sequence shows an 88.9% identity.

Please see below for the testing discount code:

DISCOUNT CODE: xxx
Expiration date: xxx
Value: xxx

I am very pleased to hear you would like to accept our offer and test ab62256 in sea urchin. This code will give you xxx off your next order before the expiration date. To redeem this offer, please submit an Abreview for sea urchin and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us.

The immunogen for the anti-phosphorylated Eph receptor antibody, ab62256, is identical to the sequence you provided, at least around Y588. I have asked the laboratory to provide a more precise alignment (I do not have access to the exact immunogen sequence).

Regarding samples, we do not have any for any of our products. Given that sea urchin is an untested species, reactivity is not covered by our guarantee. However, we do have a testing program which will provide a credit for the cost of the antibody if you first provide us with your data, regardless of whether it is positive or negative. Can you please tell me what assay you anticipate using, for example, western blotting or immunocytochemistry, or other? You will need a discount code before purchase of the antibody, which I will be happy to provide.

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Answer

Thank you for contacting us. I am sorry to hear that this antibody did not work as expected. I can certainly arrange a refund for you, but would like to gather some more information about the actual problem for our records. Please let me know: 1) What application were you using and what was the exact problem encountered (e.g. no bands/staining, high background etc) 2) What samples did you use (type of tissue/cells) and how were they prepared? 3) What is the antibody dilution and incubation time employed? 4) What was the blocking reagent? 5) What secondary antibody and detection system was used? Is it working well with other primary antibodies? 6) What controls (positive and negative) did you use? Thank you so much! I also wanted to point out that besides the option of refund, other options would be a free of charge replacement (with another antibody or an antibody to a different protein) or a credit. I look forward to hear back from you and assist you further.

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