Key features and details
- Rabbit polyclonal to Eph receptor A2+A3+A4 (phospho Y588 + Y596)
- Suitable for: ELISA, ICC/IF
- Reacts with: Human, Sea urchin
- Isotype: IgG
Product nameAnti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody
See all Eph receptor A2+A3+A4 primary antibodies
DescriptionRabbit polyclonal to Eph receptor A2+A3+A4 (phospho Y588 + Y596)
Specificityab62256 detects endogenous levels of Eph receptor A2+A3+A4 only when phosphorylated at tyrosine 588/596.
Tested applicationsSuitable for: ELISA, ICC/IFmore details
Species reactivityReacts with: Human, Sea urchin
Predicted to work with: Mouse
Synthetic phosphopeptide derived from human Eph receptor A2+A3+A4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).
- HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab62256 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab62256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
RelevanceEph receptor A2 is the receptor for members of the ephrin-A family. It binds to ephrin-A1, -A3, -A4 and -A5. Eph receptor A3 is also a receptor for members of the ephrin-A family. It binds to ephrin-A2, -A3, -A4 and -A5 and is thought to play a role in lymphoid function. Eph receptor A4 is a receptor for members of the ephrin-A family. It binds to ephrin-A1, -A4 and -A5. It binds more poorly to ephrin-A2 and -A3. It may play a role in a signal transduction process involved in hindbrain pattern formation.
Cellular localizationCell Membrane
- ECK antibody
- EPH Receptor A2 antibody
- EPH Receptor A3 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab62256 has been referenced in 4 publications.
- Mansour M et al. PTP-PEST controls EphA3 activation and ephrin-induced cytoskeletal remodelling. J Cell Sci 129:277-89 (2016). WB ; Human . PubMed: 26644181
- Neill T et al. EphA2 is a functional receptor for the growth factor progranulin. J Cell Biol 215:687-703 (2016). PubMed: 27903606
- Sabet O et al. Ubiquitination switches EphA2 vesicular traffic from a continuous safeguard to a finite signalling mode. Nat Commun 6:8047 (2015). PubMed: 26292967
- Sharonov GV et al. Point mutations in dimerization motifs of the transmembrane domain stabilize active or inactive state of the EphA2 receptor tyrosine kinase. J Biol Chem 289:14955-64 (2014). ICC/IF ; Human . PubMed: 24733396