Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody (ab62256)

Overview

  • Product name
    Anti-Eph receptor A2+A3+A4 (phospho Y588 + Y596) antibody
    See all Eph receptor A2+A3+A4 primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor A2+A3+A4 (phospho Y588 + Y596)
  • Host species
    Rabbit
  • Specificity
    ab62256 detects endogenous levels of Eph receptor A2+A3+A4 only when phosphorylated at tyrosine 588/596.
  • Tested applications
    Suitable for: ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Sea urchin
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human Eph receptor A2+A3+A4 around the phosphorylation site of tyrosine 588/596 (T-YP-V-D-P).

  • Positive control
    • HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab62256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

Images

  • ab62256 at 1/500 - 1/1000 dilution staining Eph receptor A2+A3+A4 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References

This product has been referenced in:
See all 4 Publications for this product

Customer reviews and Q&As

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Sea Urchin Cell (Embryo, Strongylocentrotus purpuratus)
Specification
Embryo, Strongylocentrotus purpuratus
Fixative
Paraformaldehyde
Permeabilization
Yes - Superblock with 0.03% TritonX-100, 1 mM Na3VO4, 1 mM NaF
Blocking step
(agent) for 30 minute(s) · Concentration: 100% · Temperature: 20°C

Dr. Robert Burke

Verified customer

Submitted Mar 28 2013

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