Anti-Eph receptor A3 antibody (ab126261)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Eph receptor A3 antibody
See all Eph receptor A3 primary antibodies -
Description
Rabbit polyclonal to Eph receptor A3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 38-335 of Human Eph receptor A3 (P29320).
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Positive control
- Juakar and Raji whole cell lysates; Human gastric carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 110 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function. -
Tissue specificity
Widely expressed. Highest level in placenta. -
Involvement in disease
Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2042 Human
- Omim: 179611 Human
- SwissProt: P29320 Human
- Unigene: 123642 Human
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Alternative names
- AW492086 antibody
- Cek4 antibody
- EC 2.7.10.1 antibody
see all
Images
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Western blot - Anti-Eph receptor A3 antibody (ab126261)All lanes : Anti-Eph receptor A3 antibody (ab126261) at 1/3000 dilution
Lane 1 : Juakat cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa
7.5% SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A3 antibody (ab126261)ab126261 at 1/500 dilution staining Eph receptor A3 in paraffin embedded Human gastric carcinoma tissue by Immunohistochemistry.
References
ab126261 has not yet been referenced specifically in any publications.
