Anti-Eph receptor A3 antibody (ab126261)
Key features and details
- Rabbit polyclonal to Eph receptor A3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor A3 antibody
See all Eph receptor A3 primary antibodies -
Description
Rabbit polyclonal to Eph receptor A3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 38-335 of Human Eph receptor A3 (P29320).
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Positive control
- Juakar and Raji whole cell lysates; Human gastric carcinoma tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab126261 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 110 kDa. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function. -
Tissue specificity
Widely expressed. Highest level in placenta. -
Involvement in disease
Defects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500]. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2042 Human
- Omim: 179611 Human
- SwissProt: P29320 Human
- Unigene: 123642 Human
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Alternative names
- AW492086 antibody
- Cek4 antibody
- EC 2.7.10.1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor A3 antibody (ab126261)ab126261 at 1/500 dilution staining Eph receptor A3 in paraffin embedded Human gastric carcinoma tissue by Immunohistochemistry.
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Western blot - Anti-Eph receptor A3 antibody (ab126261)All lanes : Anti-Eph receptor A3 antibody (ab126261) at 1/3000 dilution
Lane 1 : Juakat cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 110 kDa
7.5% SDS-PAGE
Protocols
References (0)
ab126261 has not yet been referenced specifically in any publications.
