Product nameAnti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)]
See all Eph receptor A3 primary antibodies
DescriptionRabbit monoclonal [EPR3434(N)] to Eph receptor A3 (phospho Y596)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Eph receptor A3 aa 550-650 (phospho Y596 + Y602). The exact sequence is proprietary.
Database link: P29320
- Lysate from 293 cells treated with pervanadate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 110 kDa).|
FunctionReceptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function.
Tissue specificityWidely expressed. Highest level in placenta.
Involvement in diseaseDefects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500].
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- AW492086 antibody
- Cek4 antibody
- EC 18.104.22.168 antibody
All lanes : Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] (ab191565) at 1/10000 dilution
Lane 1 : Lysate from 293 cells treated with pervanadate
Lane 2 : Lysate from untreated 293 cells
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Blocking/Dilution buffer: 5% NFDM /TBST.
ab191565 has not yet been referenced specifically in any publications.