Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3434(N)] to Eph receptor A3 (phospho Y596)
- Suitable for: WB
- Reacts with: Mouse, Human
Product nameAnti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)]
See all Eph receptor A3 primary antibodies
DescriptionRabbit monoclonal [EPR3434(N)] to Eph receptor A3 (phospho Y596)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Eph receptor A3 aa 550-650 (phospho Y596 + Y602). The exact sequence is proprietary.
Database link: P29320
- Lysate from 293 cells treated with pervanadate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab191565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 135 kDa (predicted molecular weight: 110 kDa).|
FunctionReceptor for members of the ephrin-A family. Binds to ephrin-A2, -A3, -A4 and -A5. Could play a role in lymphoid function.
Tissue specificityWidely expressed. Highest level in placenta.
Involvement in diseaseDefects in EPHA3 may be a cause of colorectal cancer (CRC) [MIM:114500].
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- AW492086 antibody
- Cek4 antibody
- EC 22.214.171.124 antibody
All lanes : Anti-Eph receptor A3 (phospho Y596) antibody [EPR3434(N)] (ab191565) at 1/10000 dilution
Lane 1 : Lysate from 293 cells treated with pervanadate
Lane 2 : Lysate from untreated 293 cells
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 110 kDa
Observed band size: 135 kDa why is the actual band size different from the predicted?
Blocking/Dilution buffer: 5% NFDM /TBST.
ab191565 has not yet been referenced specifically in any publications.