Overview

  • Product name
    Anti-Eph receptor A4/SEK antibody
    See all Eph receptor A4/SEK primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor A4/SEK
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Eph receptor A4/SEK aa 875-904 (C terminal) conjugated to keyhole limpet haemocyanin.

  • Positive control
    • NCI-H460 cell lysate
  • General notes

    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).

     This product was previously labelled as Eph receptor A4

     

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • Primary antibody notes
    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The tyrosine kinase (TK) group is mainly involved in the regulation of cell-cell interactions such as differentiation, adhesion, motility and death. There are currently about 90 TK genes sequenced, 58 are of receptor protein TK (e.g. EGFR, EPH, FGFR, PDGFR, TRK, and VEGFR families), and 32 of cytosolic TK (e.g. ABL, FAK, JAK, and SRC families).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab5396 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500.
ELISA 1/1000.
WB 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 118 kDa).

We recommend using CHAPS lysis buffer rather than RIPA, Invitrogen NuPage gel system, blotting on PVDF membrane, blocking 30' in 10% Fetal Bovine Serum, 3% BSA, 0.3% Tween 20 in 1X PBS - please see Abreview for further information (published on 24th July, 2006).

IHC-P 1/25 - 1/100.

Target

  • Function
    Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in a signal transduction process involved in hindbrain pattern formation.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Domain
    The protein kinase domain mediates interaction with NGEF/ephexin-1.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cek 8 antibody
    • CEK8 antibody
    • EK8 antibody
    • eph receptor a4 antibody
    • EPH-like kinase 8 antibody
    • EPHA4 antibody
    • EPHA4_HUMAN antibody
    • Ephrin type-A receptor 4 antibody
    • HEK 8 antibody
    • hEK8 antibody
    • Receptor protein-tyrosine kinase HEK8 antibody
    • Sek 1 antibody
    • SEK antibody
    • TYRO 1 protein tyrosine kinase antibody
    • TYRO1 antibody
    • Tyrosine-protein kinase receptor SEK antibody
    • Tyrosine-protein kinase TYRO1 antibody
    see all

Images

  • All lanes : Anti-Eph receptor A4/SEK antibody (ab5396) at 1/1000 dilution

    Lane 1 : HeLa Cell Lysate
    Lane 2 : NCI-H460 Cell Lysate
    Lane 3 : Mouse NIH3T3 Cell Lysate
    Lane 4 : Human ovary tissue Lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit IgG H+L HRP conjugated at 1/10000 dilution

    Predicted band size: 118 kDa



    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Eph receptor A4/SEK antibody (ab5396) at 1/1000 dilution + NCI-H460 whole cell lysate at 35 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 118 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab5396 at 1/500 staining rat cerebellar granual cells by ICC/IF. The cells were permeabilized with 0.1% Triton X-100, paraformaldehyde fixed and blocked with 2% serum prior to incubation with the antibody for 1 hour. A Cy3 conjugated goat anti-rabbit was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling Eph receptor A4/SEK with ab5396 at dilution 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C; heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

References

This product has been referenced in:
  • Feng L  et al. EphA4 may contribute to microvessel remodeling in the hippocampal CA1 and CA3 areas in a mouse model of temporal lobe epilepsy. Mol Med Rep 15:37-46 (2017). Read more (PubMed: 27959424) »
  • Todd KL  et al. EphA4 Regulates Neuroblast and Astrocyte Organization in a Neurogenic Niche. J Neurosci 37:3331-3341 (2017). ICC/IF ; Mouse . Read more (PubMed: 28258169) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Application
IHC - Wholemount
Sample
Common marmoset Tissue (Adult Brain)
Specification
Adult Brain

Dr. 煜欽 林

Verified customer

Submitted Jun 05 2013

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain C57Bl/6 mice)
Loading amount
30 µg
Specification
Brain C57Bl/6 mice
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3%

Abcam user community

Verified customer

Submitted Aug 23 2006

Application
Western blot
Sample
Rat Tissue lysate - whole (P0 RAT Spinal Cord)
Loading amount
30 µg
Specification
P0 RAT Spinal Cord
Blocking step
Other as blocking agent for 30 minute(s) · Concentration: 3%BSA, 10%

Abcam user community

Verified customer

Submitted Aug 14 2006

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Cerebellar granule cells)
Specification
Cerebellar granule cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% goat se
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Aug 09 2006

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