Anti-Eph receptor A5/BSK antibody (ab136116)
Key features and details
- Rabbit polyclonal to Eph receptor A5/BSK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor A5/BSK antibody
See all Eph receptor A5/BSK primary antibodies -
Description
Rabbit polyclonal to Eph receptor A5/BSK -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Eph receptor A5/BSK (internal sequence).
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Positive control
- HeLa cell lysate.
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General notes
This product was previously labelled as Eph receptor A5
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136116 was affinity-purified from rabbit antiserum by immunogenic peptide affinity-chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab136116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Predicted molecular weight: 115 kDa. |
Target
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Function
Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. Beside its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. -
Tissue specificity
Almost exclusively expressed in the nervous system in cortical neurons, cerebellar Purkinje cells and pyramidal neurons within the cortex and hippocampus. Display an increasing gradient of expression from the forebrain to hindbrain and spinal cord. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 1 Eph LBD (Eph ligand-binding) domain.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsPhosphorylated. Phosphorylation is stimulated by the ligand EFNA5. Dephosphorylation upon stimulation by glucose, inhibits EPHA5 forward signaling and results in insulin secretion. -
Cellular localization
Cell membrane. Cell projection > axon. Cell projection > dendrite. - Information by UniProt
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Database links
- Entrez Gene: 2044 Human
- Entrez Gene: 13839 Mouse
- Entrez Gene: 79208 Rat
- Omim: 600004 Human
- SwissProt: P54756 Human
- SwissProt: Q60629 Mouse
- SwissProt: P54757 Rat
- Unigene: 654492 Human
see all -
Alternative names
- Brain-specific kinase antibody
- bsk antibody
- CEK 7 antibody
see all
Images
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Western blot - Anti-Eph receptor A5/BSK antibody (ab136116)Anti-Eph receptor A5/BSK antibody (ab136116) at 1/500 dilution + HeLa cell lysate at 30 µg
Predicted band size: 115 kDa
Datasheets and documents
References (0)
ab136116 has not yet been referenced specifically in any publications.
