The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 112 kDa.
Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A2, -A3, -A4 and -A5.
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labeling Eph receptor A7 with ab176102 at 1/50 dilution which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.