Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y604) antibody (ab61791)

Overview

  • Product name
    Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y604) antibody
    See all Eph receptor B1 + Eph receptor B2 primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y604)
  • Host species
    Rabbit
  • Specificity
    ab61791 detects endogenous levels of EPHB1/2 only when phosphorylated at tyrosine 594 and tyrosine 604.
  • Tested applications
    Suitable for: WB, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human Eph receptor B1 + Eph receptor B2.

  • Positive control
    • Extracts from HepG2 cells, HuvEc cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab61791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

Target

  • Relevance
    Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor.
  • Cellular localization
    Membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • EPH B1 antibody
    • EPH B2 antibody
    • Eph tyrosine kinase 2 antibody
    • Eph tyrosine kinase 3 antibody
    • EPHB1 antibody
    • EPHB2 antibody
    • Ephrin receptor EphB1 antibody
    • Ephrin receptor EphB2 antibody
    • Ephrin type B receptor 1 antibody
    • Ephrin type B receptor 2 antibody
    • EPHT 2 antibody
    • EPHT 3 antibody
    • ERK antibody
    • Hek 5 antibody
    • Hek 6 antibody
    • Tyrosine protein kinase receptor EPH2 antibody
    • Tyrosine protein kinase receptor EPH3 antibody
    see all

Images

  • All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y604) antibody (ab61791) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HeLa whole cell lysate - overexpressing EphB2
    Lane 3 : HeLa whole cell lysate - overexpressinf EphB2-kinase dead (K661R)

    Lysates/proteins at 100000 cells per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG monoclonal at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 110 kDa
    Observed band size: 110 kDa


    Exposure time: 5 minutes


    All cells were stimulated with preclustered ephrinb1-Fc chimeric protein.

    See Abreview

  • ab61791 staining Eph receptor B1 + Eph receptor B2 (phospho Y594) in HeLa cells (overexpressing  EphB2 and stimulated for 20 minutes with preclustered efnb1-Fc chimeric protein) by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 0.1% Triton X-100) for 2 hour at 25°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody. Red - anti-EphB2 (goat), Grey - DAPI, Green - ab61791.

    See Abreview

  • All lanes : Anti-Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y604) antibody (ab61791) at 1/500 dilution

    Lane 1 : Extracts from HepG2 cells
    Lane 2 : Extracts from HepG2 cells with the immunising phosphopeptide

    Predicted band size: 110 kDa
    Observed band size: 110 kDa

  • Immunofluorescence analysis of HuvEc cells, using ab61791 at 1/500-1/1000 dilution with (+) or without (-) immunising phosphopeptide.

References

This product has been referenced in:
  • Yu SN  et al. Analgesic effects of microRNA-129-5p against bone cancer pain through the EphB1/EphrinB2 signaling pathway in mice. J Cell Biochem 120:2876-2885 (2019). Read more (PubMed: 29236320) »
  • Mimche PN  et al. EphB2 receptor tyrosine kinase promotes hepatic fibrogenesis in mice via activation of hepatic stellate cells. Sci Rep 8:2532 (2018). IHC-Fr . Read more (PubMed: 29416088) »
See all 9 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

1-2 of 2 Abreviews

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (4-20%)
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Treatment
stimulated with 1´g/ml clustered ephrinb1-Fc
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Thomas Jungas

Verified customer

Submitted Apr 11 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (HeLa cells)
Specification
HeLa cells
Permeabilization
Yes - Triton X-100 @ 0.1%
Fixative
Paraformaldehyde

Mr. Thomas Jungas

Verified customer

Submitted Mar 21 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up