Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765)
Key features and details
- Rabbit polyclonal to Eph receptor B1 + Eph receptor B2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor B1 + Eph receptor B2 antibody
See all Eph receptor B1 + Eph receptor B2 primary antibodies -
Description
Rabbit polyclonal to Eph receptor B1 + Eph receptor B2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic non-phosphopeptide derived from human Eph receptor B1 from around the phosphorylation site of tyrosine 594 (K-I-YP-I-D).
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Positive control
- Extracts from HepG2 cells, HuvEc cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab61765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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ICC/IF |
1/500 - 1/1000.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
ICC/IF
1/500 - 1/1000. |
Target
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Relevance
Ephrin receptors bind members of the ephrin ligand family. They are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. They make up the largest subgroup of the receptor tyrosine kinase (RTK) family. Eph receptor B1 (EphB1) and Eph receptor B2 (EphB2) are receptors for ephrin-B family members. Developing and adult neural tissue express nearly all of the Ephrin receptors and ephrin ligands. Ephrins and ephrin receptors also play a significant role in angiogenesis. EphB2 acts as a tumor suppressor. -
Cellular localization
Membrane; Single-pass type I membrane protein. -
Database links
- Entrez Gene: 2047 Human
- Entrez Gene: 2048 Human
- Entrez Gene: 13844 Mouse
- Entrez Gene: 270190 Mouse
- Entrez Gene: 24338 Rat
- Entrez Gene: 313633 Rat
- Omim: 600600 Human
- Omim: 600997 Human
see all -
Alternative names
- EPH B1 antibody
- EPH B2 antibody
- Eph tyrosine kinase 2 antibody
see all
Images
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HuvEc cells stained wth ab61765 at 1/500 - 1/1000, with and without the immunising peptide.
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All lanes : Anti-Eph receptor B1 + Eph receptor B2 antibody (ab61765) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells.
Lane 2 : Extracts from HepG2 cells and the immunising peptide.
Predicted band size: 110 kDa
Observed band size: 110 kDa -
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EPHB1/2 Antibody. The picture on the right is blocked with the synthesized peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab61765 has been referenced in 1 publication.
- Li L et al. Prognostic values of EphB1/B2 and p-EphB1/B2 expression in non-small cell lung cancer. Int J Clin Exp Pathol 10:10092-10101 (2017). PubMed: 31966900