Product nameAnti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody
DescriptionRabbit polyclonal to Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4
Specificityab64820 detects endogenous levels of total Eph receptor B4 protein. We have not performed cross-reactivity testing to other class B Eph receptors. The immunogen has high homology to EPHB1(100%), EPHB3 (100%), EPHB2 (92%).
Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide derived from internal region of human Eph receptor B4.
- 293 and Jurkat cell extracts.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab64820 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Our Abpromise guarantee covers the use of ab64820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Detects a band of approximately 108 kDa (predicted molecular weight: 108 kDa).|
FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
Tissue specificityPreferentially expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- CAPB antibody
- DRT antibody
- EK2 antibody
All lanes : Anti-Eph receptor B1 + Eph receptor B2 + Eph receptor B3 + Eph receptor B4 antibody (ab64820) at 1/500 dilution
Lane 1 : 293 cell extract at 30 µg
Lane 2 : Jurkat cell extract at 30 µg
Lane 3 : immunising peptide at 10 µg
Predicted band size: 108 kDa
Observed band size: 108 kDa
IHC image of ab64820 staining in human normal lymphoid formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab64820, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
This product has been referenced in:
- Protack CD et al. Eph-B4 regulates adaptive venous remodeling to improve arteriovenous fistula patency. Sci Rep 7:15386 (2017). Read more (PubMed: 29133876) »
- Model LS et al. Arterial shear stress reduces eph-b4 expression in adult human veins. Yale J Biol Med 87:359-71 (2014). IHC-P ; Human . Read more (PubMed: 25191151) »