Also tested with 5% milk as a blocking agent, but only non-specific bands at 50-60kDa were detected.
Thank you for sharing this data with us. ab64820 has previously been tested in western blot on HEK 293 cell lysates and showed a band 108kDa (please see datasheet). However, this abreview shows high background at ~50kDa, under these conditions. Abcam would welcome feedback from other customers using this product.
Abcam user community
Submitted Mar 28 2014
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