Product nameAnti-Eph receptor B1/NET antibody [EPR6457]
See all Eph receptor B1/NET primary antibodies
DescriptionRabbit monoclonal [EPR6457] to Eph receptor B1/NET
SpecificityCustomer data indicates ab129103 reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Eph receptor B1/NET aa 550-650 (phospho Y594). The exact sequence is proprietary.
- MDA-MB-435, U-87MG, NIH 3T3, COS1 and Human fetal brain cell lysates.
Product previously known as Anti-Eph receptor B1 antibody [EPR6457].
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.13 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab129103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).|
|IP||1/10 - 1/100.|
FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
Tissue specificityPreferentially expressed in brain.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- Cek 6 antibody
- EK6 antibody
- ELK antibody
All lanes : Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : U-87MG cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : COS1 cell lysate
Lane 5 : Human fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Xiong Y et al. Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury. Mol Med Rep 18:2171-2181 (2018). Read more (PubMed: 29901151) »
- Krusche B et al. EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. Elife 5:N/A (2016). Read more (PubMed: 27350048) »