Recombinant Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
![Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)](https://www.abcam.com/ps/products/129/ab129103/Images/ab129103-143092-anti-eph-receptor-b1-net-antibody-epr6457-western-blot.jpg "Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6457] to Eph receptor B1/NET
- Suitable for: WB
- Reacts with: Mouse, Human, African green monkey
Overview
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Product name
Anti-Eph receptor B1/NET antibody [EPR6457]
See all Eph receptor B1/NET primary antibodies -
Description
Rabbit monoclonal [EPR6457] to Eph receptor B1/NET -
Host species
Rabbit -
Specificity
Customer data indicates ab129103 reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence. -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human, African green monkey
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human Eph receptor B1/NET aa 550-650 (phospho Y594). The exact sequence is proprietary.
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Positive control
- MDA-MB-435, U-87MG, NIH 3T3, COS1 and Human fetal brain cell lysates.
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General notes
Product previously known as Anti-Eph receptor B1 antibody [EPR6457].
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 2.13 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6457 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system. -
Tissue specificity
Preferentially expressed in brain. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2047 Human
- Entrez Gene: 270190 Mouse
- Entrez Gene: 24338 Rat
- Omim: 600600 Human
- SwissProt: P54762 Human
- SwissProt: Q8CBF3 Mouse
- SwissProt: P09759 Rat
- Unigene: 116092 Human
see all -
Alternative names
- Cek 6 antibody
- EK6 antibody
- ELK antibody
see all
Images
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Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)All lanes : Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution
Lane 1 : MDA-MB-435 cell lysate
Lane 2 : U-87MG cell lysate
Lane 3 : NIH 3T3 cell lysate
Lane 4 : COS1 cell lysate
Lane 5 : Human fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa -
![OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)](https://www.abcam.com/ps/products/129/ab129103/Images/ab129103-143090-anti-eph-receptor-b1-net-antibody-epr6457.jpg)
OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) -
![Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)](https://www.abcam.com/ps/products/129/ab129103/Images/ab129103-4-benefits-of-recombinant-antibodies.png)
Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab129103 has been referenced in 3 publications.
- Wang L et al. Ligand-independent EphB1 signaling mediates TGF-ß-activated CDH2 and promotes lung cancer cell invasion and migration. J Cancer 11:4123-4131 (2020). PubMed: 32368295
- Xiong Y et al. Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury. Mol Med Rep 18:2171-2181 (2018). PubMed: 29901151
- Krusche B et al. EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. Elife 5:N/A (2016). PubMed: 27350048