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  1. Link

    eph-receptor-b1net-antibody-epr6457-ab129103.pdf

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Cardiovascular Angiogenesis Ephrins Eph Receptors
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RecombinantRabMAb

Recombinant Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)

  • Datasheet
Reviews (3) Submit a question References (4)

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Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
  • OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
  • Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6457] to Eph receptor B1/NET
  • Suitable for: WB
  • Reacts with: Mouse, Human, African green monkey

Conjugates logo Related conjugates and formulations

Carrier Free

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Recombinant mouse Eph receptor B1/NET protein (ab56257)
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Anti-XPNPEP2/mAPP antibody (ab97852)

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Overview

  • Product name

    Anti-Eph receptor B1/NET antibody [EPR6457]
    See all Eph receptor B1/NET primary antibodies
  • Description

    Rabbit monoclonal [EPR6457] to Eph receptor B1/NET
  • Host species

    Rabbit
  • Specificity

    Customer data indicates ab129103 reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human, African green monkey
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MDA-MB-435, U-87MG, NIH 3T3, COS1 and Human fetal brain cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 2.13 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6457
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Angiogenesis
    • Ephrins
    • Eph Receptors
    • Neuroscience
    • Neurotransmission
    • Intracellular Signaling
    • Kinases
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-Eph receptor B1/NET antibody [EPR6457] - BSA and Azide free (ab248301)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant mouse Eph receptor B1/NET protein (ab56257)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab129103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

Target

  • Function

    Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
  • Tissue specificity

    Preferentially expressed in brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P54762 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2047 Human
    • Entrez Gene: 270190 Mouse
    • Entrez Gene: 24338 Rat
    • Omim: 600600 Human
    • SwissProt: P54762 Human
    • SwissProt: Q8CBF3 Mouse
    • SwissProt: P09759 Rat
    • Unigene: 116092 Human
    • Unigene: 22897 Mouse
    • Unigene: 46606 Rat
    see all
  • Alternative names

    • Cek 6 antibody
    • EK6 antibody
    • ELK antibody
    • Elkh antibody
    • EPH receptor B1 antibody
    • Eph tyrosine kinase 2 antibody
    • EPH-like kinase 6 antibody
    • Ephb1 antibody
    • EPHB1_HUMAN antibody
    • Ephrin type B receptor 1 antibody
    • Ephrin type-B receptor 1 antibody
    • EPHT2 antibody
    • HEK 6 antibody
    • HEK6 antibody
    • NET antibody
    • Neuronally-expressed EPH-related tyrosine kinase antibody
    • soluble EPHB1 variant 1 antibody
    • Tyrosine protein kinase receptor EPH 2 antibody
    • Tyrosine-protein kinase receptor EPH-2 antibody
    see all

Images

  • Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
    Western blot - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
    All lanes : Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution

    Lane 1 : MDA-MB-435 cell lysate
    Lane 2 : U-87MG cell lysate
    Lane 3 : NIH 3T3 cell lysate
    Lane 4 : COS1 cell lysate
    Lane 5 : Human fetal brain cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 110 kDa

  • OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
    OI-RD Scanning - Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)
    Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab129103? Please let us know so that we can cite the reference in this datasheet.

ab129103 has been referenced in 4 publications.

  • Wang L  et al. Ligand-independent EphB1 signaling mediates TGF-ß-activated CDH2 and promotes lung cancer cell invasion and migration. J Cancer 11:4123-4131 (2020). PubMed: 32368295
  • Xiong Y  et al. Eph/ephrin signalling serves a bidirectional role in lipopolysaccharide-induced intestinal injury. Mol Med Rep 18:2171-2181 (2018). PubMed: 29901151
  • Kung A  et al. A Chemical-Genetic Approach to Generate Selective Covalent Inhibitors of Protein Kinases. ACS Chem Biol 12:1499-1503 (2017). PubMed: 28459525
  • Krusche B  et al. EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. Elife 5:N/A (2016). PubMed: 27350048

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunoprecipitation abreview for Anti-Eph receptor B1 / NET antibody [EPR6457]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Chicken Tissue lysate - whole (embryonic head)
Total protein in input
200 µg
Immuno-precipitation step
Other - Protein A dynabeads
Specification
embryonic head
Read More

Abcam user community

Verified customer

Submitted Jan 10 2019

Immunocytochemistry/ Immunofluorescence abreview for Anti-Eph receptor B1 / NET (phospho Y594) antibody [EPR6457]

Good
Abreviews
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Oligodendrocyte)
Permeabilization
Yes - 0.03% Trition
Specification
Oligodendrocyte
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 21 2013

Immunoprecipitation abreview for Anti-Eph receptor B1 / NET (phospho Y594) antibody [EPR6457]

Good
Abreviews
Abreviews
abreview image
Application
Immunoprecipitation
Sample
Rat Cell lysate - whole cell (Astrocytes)
Total protein in input
50 µg
Immuno-precipitation step
Protein A/G
Specification
Astrocytes
Read More

Abcam user community

Verified customer

Submitted Jan 21 2013

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