Recombinant
RabMAb

Recombinant Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103)

Overview

  • Product name
    Anti-Eph receptor B1/NET antibody [EPR6457]
    See all Eph receptor B1/NET primary antibodies
  • Description
    Rabbit monoclonal [EPR6457] to Eph receptor B1/NET
  • Host species
    Rabbit
  • Specificity
    Customer data indicates ab129103 reacts with Eph receptor B2 and B4, in addition to B1. Reactivity with receptor B3 is also expected based on 93% identity of the immunogen with the homologous receptor B3 sequence.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Eph receptor B1/NET aa 550-650 (phospho Y594). The exact sequence is proprietary.

  • Positive control
    • MDA-MB-435, U-87MG, NIH 3T3, COS1 and Human fetal brain cell lysates.
  • General notes

    Product previously known as Anti-Eph receptor B1 antibody [EPR6457].

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Receptor for members of the ephrin-B family. Binds to ephrin-B1, -B2 and -B3. Binding with the guidance cue ephrin-B2 at the optic chiasm midline redirect ventrotemporal (VT) retinal ganglion cells (RGCs) axons ipsilaterally. May be involved in cell-cell interactions in the nervous system.
    • Tissue specificity
      Preferentially expressed in brain.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cek 6 antibody
      • EK6 antibody
      • ELK antibody
      • Elkh antibody
      • EPH receptor B1 antibody
      • Eph tyrosine kinase 2 antibody
      • EPH-like kinase 6 antibody
      • Ephb1 antibody
      • EPHB1_HUMAN antibody
      • Ephrin type B receptor 1 antibody
      • Ephrin type-B receptor 1 antibody
      • EPHT2 antibody
      • HEK 6 antibody
      • HEK6 antibody
      • NET antibody
      • Neuronally-expressed EPH-related tyrosine kinase antibody
      • soluble EPHB1 variant 1 antibody
      • Tyrosine protein kinase receptor EPH 2 antibody
      • Tyrosine-protein kinase receptor EPH-2 antibody
      see all

    Images

    • All lanes : Anti-Eph receptor B1/NET antibody [EPR6457] (ab129103) at 1/1000 dilution

      Lane 1 : MDA-MB-435 cell lysate
      Lane 2 : U-87MG cell lysate
      Lane 3 : NIH 3T3 cell lysate
      Lane 4 : COS1 cell lysate
      Lane 5 : Human fetal brain cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 110 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

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    1-3 of 3 Abreviews

    Application
    Immunoprecipitation
    Sample
    Chicken Tissue lysate - whole (embryonic head)
    Total protein in input
    200 µg
    Immuno-precipitation step
    Other - Protein A dynabeads
    Specification
    embryonic head

    Abcam user community

    Verified customer

    Submitted Jan 10 2019

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Oligodendrocyte)
    Permeabilization
    Yes - 0.03% Trition
    Specification
    Oligodendrocyte
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 21 2013

    Application
    Immunoprecipitation
    Sample
    Rat Cell lysate - whole cell (Astrocytes)
    Total protein in input
    50 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    Astrocytes

    Abcam user community

    Verified customer

    Submitted Jan 21 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
    For licensing inquiries, please contact partnerships@abcam.com

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