Anti-Eph receptor B2 antibody (ab150652)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Eph receptor B2 antibody
See all Eph receptor B2 primary antibodies -
Description
Rabbit polyclonal to Eph receptor B2 -
Host species
Rabbit -
Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Hamster, Cow, Turkey, Pig, Xenopus laevis, Pufferfish, Zebrafish, Gorilla, Opossum, Common marmoset, Elephant
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Immunogen
Synthetic peptide, corresponding to 15 amino acids from an internal region of Human Eph receptor B2 (AAB94602.1).
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Positive control
- Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab150652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 7 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor. -
Tissue specificity
Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain. -
Involvement in disease
Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 396513 Chicken
- Entrez Gene: 535137 Cow
- Entrez Gene: 2048 Human
- Entrez Gene: 13844 Mouse
- Entrez Gene: 100519549 Pig
- Entrez Gene: 313633 Rat
- Entrez Gene: 399363 Xenopus laevis
- Entrez Gene: 562176 Zebrafish
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Alternative names
- cek5 antibody
- Developmentally regulated EPH related tyrosine kinase antibody
- DRT antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B2 antibody (ab150652)Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labelling Eph receptor B2 with ab150652 at 7 µg/ml.
Protocols
References
This product has been referenced in:
- Mather Q et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »
