Overview

  • Product name
    Anti-Eph receptor B2 antibody
    See all Eph receptor B2 primary antibodies
  • Description
    Rabbit polyclonal to Eph receptor B2
  • Host species
    Rabbit
  • Specificity
    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Chicken, Hamster, Cow, Turkey, Pig, Xenopus laevis, Pufferfish, Zebrafish, Gorilla, Opossum, Common marmoset, Elephant
  • Immunogen

    Synthetic peptide, corresponding to 15 amino acids from an internal region of Human Eph receptor B2 (AAB94602.1).

  • Positive control
    • Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab150652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 7 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Target

  • Function
    Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.
  • Tissue specificity
    Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
  • Involvement in disease
    Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • cek5 antibody
    • Developmentally regulated EPH related tyrosine kinase antibody
    • DRT antibody
    • EK5 antibody
    • ELK related protein tyrosine kinase antibody
    • Eph receptor B2 antibody
    • EPH tyrosine kinase 3 antibody
    • EPH-like kinase 5 antibody
    • EPHB2 antibody
    • EPHB2_HUMAN antibody
    • Ephrin type B receptor 2 antibody
    • Ephrin type-B receptor 2 antibody
    • EPHT 3 antibody
    • ERK antibody
    • ETECK antibody
    • hEK5 antibody
    • Nuk antibody
    • Prkm 5 antibody
    • Receptor protein tyrosine kinase HEK 5 antibody
    • Renal carcinoma antigen NY-REN-47 antibody
    • Sek 3 antibody
    • Tyro 5 antibody
    • Tyrosine protein kinase receptor CEK 5 antibody
    • Tyrosine protein kinase receptor EPH 3 antibody
    • Tyrosine protein kinase receptor QEK 5 antibody
    • Tyrosine-protein kinase receptor EPH-3 antibody
    • Tyrosine-protein kinase TYRO5 antibody
    see all

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labelling Eph receptor B2 with ab150652 at 7 µg/ml.

References

This product has been referenced in:
  • Mather Q  et al. A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas. Mol Clin Oncol 7:315-321 (2017). Read more (PubMed: 28781809) »
See 1 Publication for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Rat Cell lysate - whole cell (Mixed primary culture from Enteric nervous system)
Gel Running Conditions
Reduced Denaturing (4-12% Bis Tris acrylamide)
Loading amount
15 µg
Specification
Mixed primary culture from Enteric nervous system
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Oct 23 2017

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