Anti-Eph receptor B2 antibody (ab216629)
Key features and details
- Rabbit polyclonal to Eph receptor B2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Eph receptor B2 antibody
See all Eph receptor B2 primary antibodies -
Description
Rabbit polyclonal to Eph receptor B2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide aa 100-200 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P29323 -
Positive control
- IHC-P: Human kidney tissue. WB: Mouse testis lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 117 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 117 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor. -
Tissue specificity
Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain. -
Involvement in disease
Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2048 Human
- Entrez Gene: 13844 Mouse
- Omim: 600997 Human
- SwissProt: P29323 Human
- SwissProt: P54763 Mouse
- Unigene: 523329 Human
- Unigene: 250981 Mouse
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Alternative names
- cek5 antibody
- Developmentally regulated EPH related tyrosine kinase antibody
- DRT antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Eph receptor B2 using ab216629 at 1/200, followed by secondary detection and DAB staining.
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Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Eph receptor B2 using ab216629 at 1/200, followed by secondary detection and DAB staining.
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Anti-Eph receptor B2 antibody (ab216629) at 1/300 dilution + mouse testis lysate
Predicted band size: 117 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab216629 has been referenced in 2 publications.
- Vilallongue N et al. Guidance landscapes unveiled by quantitative proteomics to control reinnervation in adult visual system. Nat Commun 13:6040 (2022). PubMed: 36229455
- Wang S et al. Circular RNA RBM33 contributes to extracellular matrix degradation via miR-4268/EPHB2 axis in abdominal aortic aneurysm. PeerJ 9:e12232 (2021). PubMed: 34820156