Overview

  • Product name

    Anti-Eph receptor B2 antibody
    See all Eph receptor B2 primary antibodies
  • Description

    Rabbit polyclonal to Eph receptor B2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide aa 100-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    I PSVPGSCKET FNLYYYEADF DSATKTFPNW MENPWVKVDT IAADESFSQV


    Database link: P29323

  • Positive control

    • IHC-P: Human kidney tissue. WB: Mouse testis lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab216629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 117 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Receptor for members of the ephrin-B family. Phosphorylates ARHGEF15, leading to its ubiquitination and degradation by the proteasome which promotes EFNB1-dependent synapse formation. Can function in aspects of retinal ganglion cell axon guidance to the optic disk even when lacking its tyrosine kinase domain. Acts as a tumor suppressor.
  • Tissue specificity

    Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
  • Involvement in disease

    Defects in EPHB2 may be a cause of susceptibility to prostate cancer (PC) [MIM:176807]. It is a malignancy originating in tissues of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma. Note=EPHB2 mutations have been found in a prostate cancer cell line derived from a brain metastasis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • cek5 antibody
    • Developmentally regulated EPH related tyrosine kinase antibody
    • DRT antibody
    • EK5 antibody
    • ELK related protein tyrosine kinase antibody
    • Eph receptor B2 antibody
    • EPH tyrosine kinase 3 antibody
    • EPH-like kinase 5 antibody
    • EPHB2 antibody
    • EPHB2_HUMAN antibody
    • Ephrin type B receptor 2 antibody
    • Ephrin type-B receptor 2 antibody
    • EPHT 3 antibody
    • ERK antibody
    • ETECK antibody
    • hEK5 antibody
    • Nuk antibody
    • Prkm 5 antibody
    • Receptor protein tyrosine kinase HEK 5 antibody
    • Renal carcinoma antigen NY-REN-47 antibody
    • Sek 3 antibody
    • Tyro 5 antibody
    • Tyrosine protein kinase receptor CEK 5 antibody
    • Tyrosine protein kinase receptor EPH 3 antibody
    • Tyrosine protein kinase receptor QEK 5 antibody
    • Tyrosine-protein kinase receptor EPH-3 antibody
    • Tyrosine-protein kinase TYRO5 antibody
    see all

Images

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Eph receptor B2 using ab216629 at 1/200, followed by secondary detection and DAB staining.

  • Immunohistochemical analysis of formalin-fixed and paraffin embedded human kidney tissue labeling Eph receptor B2 using ab216629 at 1/200, followed by secondary detection and DAB staining.

  • Anti-Eph receptor B2 antibody (ab216629) at 1/300 dilution + mouse testis lysate

    Predicted band size: 117 kDa

References

ab216629 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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