Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8280] to Eph receptor B3
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Product nameAnti-Eph receptor B3 antibody [EPR8280]
See all Eph receptor B3 primary antibodies
DescriptionRabbit monoclonal [EPR8280] to Eph receptor B3
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Eph receptor B3 aa 100-200 (extracellular). The exact sequence is proprietary.
- WB: Jurkat, HepG2, HeLa, MOLT4, NIH3T3, Rat-1 and C6 cell lysates. Rat spinal cord tissue lysate; ICC/IF: Jurkat and HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Dissociation constant (KD)KD = 5.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab133742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).
For unpurified use at 1/1000 - 1/10000.
For unpurified use at 1/100 - 1/250.
FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B1 and -B2.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- Cek10 antibody
- EK2 antibody
- Embryonic kinase 2 antibody
All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution (Purified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates
Lane 2 : MOLT-4 (Human lymphoblastic leukemia T lymphoblast) whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 5 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 7 : Rat spinal cord lysates
Lane 8 : Rat-1 (Rat embryonic fibroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 110 kDa
Observed band size: 110 kDa
Blocking/Diluting buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Eph receptor B3 with Purified ab133742 at 1/50 dilution (5.14 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution (unpurified)
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa
Immunocytochemical analysis of HeLa cells labelling Eph receptor B3 with unpurified ab133742 at 1/100.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab133742 has been referenced in 3 publications.
- Krusche B et al. EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. Elife 5:N/A (2016). PubMed: 27350048
- Murphy M et al. TCF7L1 Modulates Colorectal Cancer Growth by Inhibiting Expression of the Tumor-Suppressor Gene EPHB3. Sci Rep 6:28299 (2016). PubMed: 27333864
- Lin B et al. Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration. Nat Commun 6:6619 (2015). PubMed: 25851023