Product nameAnti-Eph receptor B3 antibody [EPR8280]
See all Eph receptor B3 primary antibodies
DescriptionRabbit monoclonal [EPR8280] to Eph receptor B3
Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to a region within the extracellular domain of Human Eph receptor B3 (P54753)
- Jurkat, HepG2, HeLa, MOLT4, NIH3T3 cell lysate; HeLa cells
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 5.70 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133742 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionReceptor for members of the ephrin-B family. Binds to ephrin-B1 and -B2.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain.
- Information by UniProt
- Cek10 antibody
- EK2 antibody
- Embryonic kinase 2 antibody
All lanes : Anti-Eph receptor B3 antibody [EPR8280] (ab133742) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : NIH3T3 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 110 kDa
Immunocytochemical analysis of HeLa cells labelling Eph receptor B3 with ab133742 at 1/100.
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
This product has been referenced in:
- Krusche B et al. EphrinB2 drives perivascular invasion and proliferation of glioblastoma stem-like cells. Elife 5:N/A (2016). Read more (PubMed: 27350048) »
- Lin B et al. Interplay between chemotaxis and contact inhibition of locomotion determines exploratory cell migration. Nat Commun 6:6619 (2015). Read more (PubMed: 25851023) »