Anti-Eph receptor B4/HTK antibody [VEB4-7E4] (ab106130)
Key features and details
- Rat monoclonal [VEB4-7E4] to Eph receptor B4/HTK
- Suitable for: ELISA, IHC-Fr, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Eph receptor B4/HTK antibody [VEB4-7E4]
See all Eph receptor B4/HTK primary antibodies -
Description
Rat monoclonal [VEB4-7E4] to Eph receptor B4/HTK -
Host species
Rat -
Specificity
ab106130 marks venous endothelial cells, but not arterial endothelial cells in B16 melanoma cells. -
Tested applications
Suitable for: ELISA, IHC-Fr, ICC/IF, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
The details of the immunogen for this antibody are not available.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
0.2 µm filtered antibody solution -
Clonality
Monoclonal -
Clone number
VEB4-7E4 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
Target
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Function
Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development. -
Tissue specificity
Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Post-translational
modificationsAutophosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13846 Mouse
- SwissProt: P54761 Mouse
- Unigene: 34533 Mouse
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Alternative names
- Ephb4 antibody
- EPHB4_HUMAN antibody
- Ephrin receptor EphB4 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab106130 has been referenced in 1 publication.
- Xue C et al. Matrix stiffness regulates arteriovenous differentiation of endothelial progenitor cells during vasculogenesis in nude mice. Cell Prolif 52:e12557 (2019). PubMed: 30485569