Recombinant
RabMAb

Recombinant Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

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Overview

  • Product name

    Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free
    See all Eph receptor B4/HTK primary antibodies

  • Description

    Rabbit monoclonal [EPR23221-54] to Eph receptor B4/HTK - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human Eph receptor B4/HTK aa 850-950. The exact sequence is proprietary.
    Database link: P54760

  • Positive control

    • WB: HT-29, HUVEC, HCT 116 and MCF7 lysates. IP: HT-29 and HUVEC whole cell lysates.

  • General notes

    ab269867 is the carrier-free version of ab254300. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269867 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.?? 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab269867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 108 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.

    • Tissue specificity

      Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.

    • Post-translational
      modifications

      Autophosphorylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Ephb4 antibody
        • EPHB4_HUMAN antibody
        • Ephrin receptor EphB4 antibody
        • Ephrin type-B receptor 4 antibody
        • Hepatoma Transmembrane Kinase antibody
        • HTK antibody
        • MYK 1 antibody
        • MYK1 antibody
        • TYRO 11 antibody
        • TYRO11 antibody
        • Tyrosine-protein kinase receptor HTK antibody
        • Tyrosine-protein kinase TYRO11 antibody
        see all

      Images

      • Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)
        Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

        Eph receptor B4 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab254300 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254300 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

        Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10ug

        Lane 2: ab254300 IP in HUVEC whole cell lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254300 in HUVEC whole cell lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 8 seconds.

        Cleaved intracellular domain (ICD) was observed at around 47kDa. (PMID: 24854540).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254300).

      • Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)
        Immunoprecipitation - Anti-Eph receptor B4/HTK antibody [EPR23221-54] - BSA and Azide free (ab269867)

        Eph receptor B4 was immunoprecipitated from 0.35 mg HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate with ab254300 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254300 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

        Lane 1: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate 10ug

        Lane 2: ab254300 IP in HT-29 whole cell lysate

        Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254300 in HT-29 whole cell lysate

        Blocking and dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: 8 seconds.

        Cleaved intracellular domain (ICD) was observed at around 47kDa. (PMID: 24854540).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254300).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References

      Publishing research using ab269867? Please let us know so that we can cite the reference in this datasheet.

      ab269867 has not yet been referenced specifically in any publications.

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