Recombinant
RabMAb

Recombinant Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free (ab269369)

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Overview

  • Product name

    Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free
    See all Eph receptor B4/HTK primary antibodies

  • Description

    Rabbit monoclonal [EPR23222-24] to Eph receptor B4/HTK - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment within Human Eph receptor B4/HTK aa 1-550. The exact sequence is proprietary.
    Database link: P54760

  • Positive control

    • WB: HT-29, HUVEC, HCT116, MCF7, T47D, PC-3, NIH:OVCAR-3, SK-BR-3, GR-M, 4T1 and NIH/3T3 lysates. IHC-P: Human colon, Human colon cancer and Human breast cancer tissues.

  • General notes

    ab269369 is the carrier-free version of ab254301. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269369 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab269369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 108 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.

    • Target

    • Function

      Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.

    • Tissue specificity

      Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.

    • Sequence similarities

      Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.

    • Post-translational
      modifications

      Autophosphorylated.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Ephb4 antibody
        • EPHB4_HUMAN antibody
        • Ephrin receptor EphB4 antibody
        • Ephrin type-B receptor 4 antibody
        • Hepatoma Transmembrane Kinase antibody
        • HTK antibody
        • MYK 1 antibody
        • MYK1 antibody
        • TYRO 11 antibody
        • TYRO11 antibody
        • Tyrosine-protein kinase receptor HTK antibody
        • Tyrosine-protein kinase TYRO11 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free (ab269369)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free (ab269369)

        Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling Eph receptor B4 with ab254301 at 1/1000 dilution (0.545ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at a ready to use dilution. Mainly membranous staining in cancer cells of human breast cancer (PMID: 16816380) is observed. The section was incubated with ab255611 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254301).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free (ab269369)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free (ab269369)

        Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Eph receptor B4 with ab254301 at 1/1000 dilution (0.545ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at a ready to use dilution. Positive staining in cancer cells of human colon cancer (PMID: 19366806) is observed. The section was incubated with ab255611 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254301).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free (ab269369)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eph receptor B4/HTK antibody [EPR23222-24] - BSA and Azide free (ab269369)

        Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Eph receptor B4 with ab254301 at 1/1000 dilution (0.545ug/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at a ready to use dilution. Weak positive staining in base of crypts of human colon (PMID:19366806) is observed. The section was incubated with ab255611 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

        Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution.

        Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254301).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References

      Publishing research using ab269369? Please let us know so that we can cite the reference in this datasheet.

      ab269369 has not yet been referenced specifically in any publications.

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