Overview

  • Product name

    Anti-Eph receptor B4/HTK antibody [RM0056-6A14]
    See all Eph receptor B4/HTK primary antibodies
  • Description

    Rat monoclonal [RM0056-6A14] to Eph receptor B4/HTK
  • Host species

    Rat
  • Specificity

    ab73259 is specific for mouse Eph receptor B4/HTK. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 and EphB6

  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse Eph receptor B4/HTK (extracellular).

  • General notes

     This product was previously labelled as Eph receptor B4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab73259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 109 kDa.
IHC-P 1/100 - 1/200.

Perform enzymatic antigen retrieval with 20 ug/ml proteinase K before commencing with IHC protocol.

Target

  • Function

    Receptor for members of the ephrin-B family. Binds to ephrin-B2. May have a role in events mediating differentiation and development.
  • Tissue specificity

    Abundantly expressed in placenta and in a range of primary tissues and malignant cell lines. Expressed in fetal, but not adult, brain, and in primitive and myeloid, but not lymphoid, hematopoietic cells.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 1 protein kinase domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Post-translational
    modifications

    Autophosphorylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ephb4 antibody
    • EPHB4_HUMAN antibody
    • Ephrin receptor EphB4 antibody
    • Ephrin type-B receptor 4 antibody
    • Hepatoma Transmembrane Kinase antibody
    • HTK antibody
    • MYK 1 antibody
    • MYK1 antibody
    • TYRO 11 antibody
    • TYRO11 antibody
    • Tyrosine-protein kinase receptor HTK antibody
    • Tyrosine-protein kinase TYRO11 antibody
    see all

Images

  • ab73259 at 1/200 dilution, staining Eph receptor B4/HTK in LPS treated mouse kidney tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections).

  • ab73259 staining the Eph receptor B4/HTK in Mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-rat polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Munro DAD  et al. Refuting the hypothesis that semaphorin-3f/neuropilin-2 exclude blood vessels from the cap mesenchyme in the developing kidney. Dev Dyn 246:1047-1056 (2017). Read more (PubMed: 28929539) »
  • Chen II  et al. EphrinB2 regulates the emergence of a hemogenic endothelium from the aorta. Sci Rep 6:27195 (2016). ICC/IF ; Mouse . Read more (PubMed: 27250641) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lymph node)
Antigen retrieval step
None
Permeabilization
No
Specification
Lymph node
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Sep 10 2010

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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