Anti-Eph receptor B6 antibody [RM0057-9B17] (ab73260)
Key features and details
- Rat monoclonal [RM0057-9B17] to Eph receptor B6
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG2
Overview
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Product name
Anti-Eph receptor B6 antibody [RM0057-9B17]
See all Eph receptor B6 primary antibodies -
Description
Rat monoclonal [RM0057-9B17] to Eph receptor B6 -
Host species
Rat -
Specificity
ab73260 is specific for mouse Eph receptor B6. It does not cross-react with recombinant mouse EphA2, EphA3, EphA4, EphA6, EphA7 EphA8, EphB2, EphB3, and EphB4 -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant mouse Eph receptor B6 extracellular domain
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab73260 was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with recombinant mouse Eph receptor B6 extracellular domain). -
Clonality
Monoclonal -
Clone number
RM0057-9B17 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab73260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200.
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Notes |
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IHC-P
1/50 - 1/200. |
Target
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Function
Kinase-defective receptor for members of the ephrin-B family. Binds to ephrin-B1 and ephrin-B2. Modulates cell adhesion and migration by exerting both positive and negative effects upon stimulation with ephrin-B2. Inhibits JNK activation, T cell receptor-induced IL-2 secretion and CD25 expression upon stimulation with ephrin-B2. -
Tissue specificity
Expressed in brain. Expressed in non invasive breast carcinoma cell lines (at protein level). Strong expression in brain and pancreas, and weak expression in other tissues, such as heart, placenta, lung, liver, skeletal muscle and kidney. Expressed in breast non invasive tumors but not in metastatic lesions. Isoform 3 is expressed in cell lines of glioblastomas, anaplastic astrocytomas, gliosarcomas and astrocytomas. Isoform 3 is not detected in normal tissues. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
Contains 2 fibronectin type-III domains.
Contains 1 protein kinase domain.
Contains 1 SAM (sterile alpha motif) domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. Its extracellular domain is capable of promoting cell adhesion and migration in response to low concentrations of ephrin-B2, but its cytoplasmic domain is essential for cell repulsion and inhibition of migration induced by high concentrations of ephrin-B2. -
Post-translational
modificationsLigand-binding increases phosphorylation on tyrosine residues. Phosphorylation on tyrosine residues is mediated by transphosphorylation by the catalytically active EPHB1 in a ligand-independent manner. Tyrosine phosphorylation of the receptor may act as a switch on the functional transition from cell adhesion/attraction to de-adhesion/repulsion. -
Cellular localization
Secreted and Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13848 Mouse
- SwissProt: O08644 Mouse
- Unigene: 271976 Mouse
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Alternative names
- EPH receptor B6 antibody
- Ephb6 antibody
- EPHB6_HUMAN antibody
see all
Images
Datasheets and documents
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Datasheet download
References (0)
ab73260 has not yet been referenced specifically in any publications.