Overview

  • Product name
    Anti-EPHA8 antibody
  • Description
    Goat polyclonal to EPHA8
  • Host species
    Goat
  • Specificity
    Anti-Mouse EphA8 recognizes recombinant mouse EphA8 by immunoblotting and ELISA. The antibody shows approximately 5% cross-reactivity with recombinant rat EphA5, recombinant mouse EphA2, recombinant mouse EphA3, recombinant mouse EphA6, recombinant mouse EphA7, and recombinant human EphA1.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse EPHA8.

  • General notes

    Previously labelled as Eph receptor A8. 

Properties

Applications

Our Abpromise guarantee covers the use of ab10615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: Use at a concentration of 0.5 - 1 µg/ml. For ELISAs, a working antibody concentration of 0.5-1.0 µg/ml is recommended. The detection limit for recombinant mouse EphA8 is approximately 0.3 ng/well.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 120 kDa). The detection limit for recombinant mouse EphA8 is approximately 2 ng/lane under non-reducing and reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Receptor for members of the ephrin-A family.
    • Sequence similarities
      Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 1 protein kinase domain.
      Contains 1 SAM (sterile alpha motif) domain.
    • Post-translational
      modifications
      Phosphorylation on Tyr-616 is critical for association with FYN.
      Phosphorylation on Tyr-839 modulates tyrosine kinase activity.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 2.7.1.112 antibody
      • EEK antibody
      • EK3 antibody
      • EPH and ELK related kinase antibody
      • EPH like kinase 3 antibody
      • Eph receptor A8 antibody
      • EPH- and ELK-related kinase antibody
      • EPH-like kinase 3 antibody
      • Epha8 antibody
      • EPHA8_HUMAN antibody
      • Ephrin Type A Receptor 8 Precursor antibody
      • Ephrin type-A receptor 8 antibody
      • HEK3 antibody
      • KIAA1459 antibody
      • Tyrosine-protein kinase receptor EEK antibody
      • Tyrosylprotein Kinase antibody
      see all

    References

    ab10615 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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