Key features and details
- Rabbit polyclonal to Ephexin-1 - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Ephexin-1 antibody - C-terminal
See all Ephexin-1 primary antibodies
DescriptionRabbit polyclonal to Ephexin-1 - C-terminal
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Chicken
Synthetic peptide coupled to a carrier protein, corresponding to C-terminal amino acids of Mouse Ephexin-1 (UniProt: Q8CHT1).
- A431, Jurkat and HUVEC cell lysates; Rat PC12 cell lysate; Mouse brain tissue lysate; Mouse C2C12 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab157593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 82 kDa.|
FunctionActs as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
Tissue specificityHighly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe DH domain and the PH domain are both required to mediate interaction with EPHA4.
modificationsSrc-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
Cellular localizationCytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones.
- Information by UniProt
- ARHGEF27 antibody
- Eph receptor-interacting exchange protein antibody
- Eph-interacting exchange protein antibody
All lanes : Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution
Lane 1 : A431 cell lysate
Lane 2 : A431 cell lysate, treated with pervanadate (1mM; 30 mins)
Predicted band size: 82 kDa
Immunofluorescent analysis of Mouse C2C12 cells, labeling Ephexin-1 with ab157593 at 1/50 dilution. Right image shows cells treated with pervanadate.
ab157593 has not yet been referenced specifically in any publications.