Overview

  • Product name

    Anti-Ephexin-1 antibody - C-terminal
    See all Ephexin-1 primary antibodies
  • Description

    Rabbit polyclonal to Ephexin-1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken
  • Immunogen

    Synthetic peptide coupled to a carrier protein, corresponding to C-terminal amino acids of Mouse Ephexin-1 (UniProt: Q8CHT1).

  • Positive control

    • A431, Jurkat and HUVEC cell lysates; Rat PC12 cell lysate; Mouse brain tissue lysate; Mouse C2C12 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab157593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 82 kDa.
ICC/IF 1/50.

Target

  • Function

    Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
  • Tissue specificity

    Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
  • Sequence similarities

    Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • Domain

    The DH domain and the PH domain are both required to mediate interaction with EPHA4.
  • Post-translational
    modifications

    Src-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
  • Cellular localization

    Cytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARHGEF27 antibody
    • Eph receptor-interacting exchange protein antibody
    • Eph-interacting exchange protein antibody
    • EPHEXIN antibody
    • Ephexin-1 antibody
    • Guanine nucleotide exchange factor, neuronal antibody
    • Neuronal guanine nucleotide exchange factor antibody
    • Ngef antibody
    • NGEF_HUMAN antibody
    see all

Images

  • All lanes : Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : A431 cell lysate, treated with pervanadate (1mM; 30 mins)

    Predicted band size: 82 kDa

  • Immunofluorescent analysis of Mouse C2C12 cells, labeling Ephexin-1 with ab157593 at 1/50 dilution. Right image shows cells treated with pervanadate.

References

ab157593 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (spinal cord, motor neuron)
Permeabilization
No
Specification
spinal cord, motor neuron
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted May 16 2016

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