The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 82 kDa.
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
Immunofluorescent analysis of mouse C2C12 cells labeling Exphexin-1 with ab157593 (C-terminal region) (upper images) or ab157594 (Tyr-87) (lower images) at 1/50 dilution, detected using appropriate secondary antibodies conjugated to Cy3. Cells in the right images were treated with pervanadate.
Western blot - Anti-Ephexin-1 (phospho Y87) antibody (ab157594)