Anti-Ephexin-1 (phospho Y87) antibody (ab157594)
Key features and details
- Rabbit polyclonal to Ephexin-1 (phospho Y87)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Ephexin-1 (phospho Y87) antibody
See all Ephexin-1 primary antibodies -
Description
Rabbit polyclonal to Ephexin-1 (phospho Y87) -
Host species
Rabbit -
Specificity
The specific band detected in WB is removed by alkaline phosphatase treatment. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken -
Immunogen
Synthetic peptide corresponding to Mouse Ephexin-1 (phospho Y87).
Database link: Q8CHT1 -
Positive control
- A431 cell lysate; Mouse C2C12 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab157594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000. Predicted molecular weight: 82 kDa.
|
ICC/IF |
1/200.
|
Notes |
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WB
1/1000. Predicted molecular weight: 82 kDa. |
ICC/IF
1/200. |
Target
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Function
Acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension. -
Tissue specificity
Highly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung. -
Sequence similarities
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain. -
Domain
The DH domain and the PH domain are both required to mediate interaction with EPHA4. -
Post-translational
modificationsSrc-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA. -
Cellular localization
Cytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones. - Information by UniProt
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Database links
- Entrez Gene: 424744 Chicken
- Entrez Gene: 25791 Human
- Entrez Gene: 53972 Mouse
- Entrez Gene: 246217 Rat
- Omim: 605991 Human
- SwissProt: Q8N5V2 Human
- SwissProt: Q8CHT1 Mouse
- SwissProt: Q5BKC9 Rat
see all -
Alternative names
- ARHGEF27 antibody
- Eph receptor-interacting exchange protein antibody
- Eph-interacting exchange protein antibody
see all
Images
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Immunofluorescent analysis of mouse C2C12 cells labeling Exphexin-1 with ab157593 (C-terminal region) (upper images) or ab157594 (Tyr-87) (lower images) at 1/50 dilution, detected using appropriate secondary antibodies conjugated to Cy3. Cells in the right images were treated with pervanadate.
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Lanes 1-2 : Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution
Lanes 3-4 : Anti-Ephexin-1 (phospho Y87) antibody (ab157594) at 1/1000 dilution
Lanes 1 & 3 : Lysate from A431 cells
Lanes 2 & 4 : Lysate from A431 cells treated
with pervanadate (1 mM) for 30 min.
Predicted band size: 82 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab157594 has been referenced in 1 publication.
- Fan R et al. Critical role of EphA4 in early brain injury after subarachnoid hemorrhage in rat. Exp Neurol 296:41-48 (2017). PubMed: 28698029