Key features and details
- Rabbit polyclonal to Ephexin-1 (phospho Y87)
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Ephexin-1 (phospho Y87) antibody
See all Ephexin-1 primary antibodies
DescriptionRabbit polyclonal to Ephexin-1 (phospho Y87)
SpecificityThe specific band detected in WB is removed by alkaline phosphatase treatment.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken
Synthetic phosphopeptide coupled to a carrier protein, corresponding to residues surrounding tyrosine 87 of Mouse Ephexin-1 (UniProt ID: Q8CHT1).
- A431 cell lysate; Mouse C2C12 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab157594 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 82 kDa.|
FunctionActs as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity switches toward RHOA resulting in its activation. Activated RHOA promotes cone retraction at the expense of RAC1-and CDC42-stimulated growth cone extension.
Tissue specificityHighly expressed in brain specifically in caudate nucleus and to a lower extent in amygdala and hippocampus. Also detected in lung.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 SH3 domain.
DomainThe DH domain and the PH domain are both required to mediate interaction with EPHA4.
modificationsSrc-dependent phosphorylation at Tyr-179 upon EPHA4 activation increases the guanine exchange factor activity toward RHOA.
Cellular localizationCytoplasm. Membrane. Cell projection > growth cone. Associated with membranes. Localizes to axonal growth cones.
- Information by UniProt
- ARHGEF27 antibody
- Eph receptor-interacting exchange protein antibody
- Eph-interacting exchange protein antibody
Immunofluorescent analysis of mouse C2C12 cells labeling Exphexin-1 with ab157593 (C-terminal region) (upper images) or ab157594 (Tyr-87) (lower images) at 1/50 dilution, detected using appropriate secondary antibodies conjugated to Cy3. Cells in the right images were treated with pervanadate.
Lanes 1-2 : Anti-Ephexin-1 antibody - C-terminal (ab157593) at 1/1000 dilution
Lanes 3-4 : Anti-Ephexin-1 (phospho Y87) antibody (ab157594) at 1/1000 dilution
Lanes 1 & 3 : Lysate from A431 cells
Lanes 2 & 4 : Lysate from A431 cells treated
with pervanadate (1 mM) for 30 min.
Predicted band size: 82 kDa
ab157594 has been referenced in 1 publication.
- Fan R et al. Critical role of EphA4 in early brain injury after subarachnoid hemorrhage in rat. Exp Neurol 296:41-48 (2017). PubMed: 28698029